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Series GSE118055 Query DataSets for GSE118055
Status Public on Aug 21, 2018
Title Single cell changes in DBZ or DMSO treated cells derived from Apc-mutant organoid cultures
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We made intestinal organoid cultures from Villin-CreERT2;Apcflox/flox mice. Organoids were subjected to 4-OH-Tam treatment, whereafter Apc mutant organoids were selected in R-Spondin free medium. DBZ or DMSO treatment was started on day 3. On day 8, organoids were dissociated to obtain single cells and analyzed using the Chromium Single-cell 3'RNA-sequencing system
Overall design We analyzed in total 2 samples (1 DBZ and 1 DMSO), both samples contained 3000 cells
Contributor(s) Högström J, Alitalo K
Citation(s) 30154153
Submission date Aug 02, 2018
Last update date Mar 08, 2019
Contact name Jenny Hogstrom
Organization name University of Helsinki
Street address Haartmaninkatu 8
City Helsinki
ZIP/Postal code 00290
Country Finland
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM3317831 DBZ-treated Apc mutant organoids
GSM3317832 DMSO-treated Apc mutant organoids
BioProject PRJNA484197
SRA SRP156203

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118055_normalized_counts.txt.gz 57.2 Mb (ftp)(http) TXT
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Processed data are available on Series record

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