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Series GSE11792 Query DataSets for GSE11792
Status Public on Nov 30, 2008
Title Human Skin: Before and 8 weeks after Isotretinoin Treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The pathogenesis of acne has been linked to multiple factors such as increased sebum production, inflammation, follicular hyperkeratinization, and the action of Propionibacterium acnes within the follicle. 13-cis Retinoic Acid (13-cis RA, isotretinoin) is the most potent agent in acne treatment. Surprisingly, its mechanism of action in acne is still unknown. Gene expression profiling of skin from 8 patients treated with isotretinoin was performed to gain insights into its mechanism of action. Skin biopsies were obtained from the patients at baseline and at 8 weeks isotretinoin treatment. Gene array expression profiling was conducted using Affymetrix HG-U133A 2.0 arrays in order to examine changes in gene expression as a result of treatment. After treatment, 784 genes were significantly changed: 197 up-regulated and 587 down-regulated. The majority of genes that were up-regulated at 8 weeks encode structural proteins of the extracellular matrix such as collagens, fibulin and fibronectin. The preponderance of genes that were down-regulated at 8 weeks are involved in the metabolism of steroids, cholesterol and fatty acids.

Keywords: drug treatment effects
Overall design Total 16 chips: 8 baseline/before isotretinoin and 8 after 8 weeks isotretinoin treatment.
Contributor(s) Thiboutot DM
Citation(s) 18987667, 20436886
Submission date Jun 16, 2008
Last update date Sep 25, 2019
Contact name Diane M Thiboutot
Organization name The Pennsylvannia State University College of Medicine
Department Department of Dermatology; Jake Gittlen Res. Fnd.
Street address 500 University Drive
City Hershey
State/province PA
ZIP/Postal code 17033
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (16)
GSM298370 Pat 1_skin_baseline
GSM298371 Pat 1_skin_treated
GSM298372 Pat 2_skin_baseline
BioProject PRJNA105993

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Supplementary file Size Download File type/resource
GSE11792_RAW.tar 50.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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