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Series GSE117684 Query DataSets for GSE117684
Status Public on Dec 19, 2020
Title Whole-genome mapping of RUNX1, CBFA2T3, H3K4me1, H3K4me3, H3k27me3 and H3K27ac in pre-B lymphoblast models
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Genome binding/occupancy profiling of Runt Related Transcription Factor 1 (RUNX1), CBFA2T3 (ETO2, MRG16) and histone marks by high throughput sequencing. RUNX1 and CBFA2T3 are found overexpressed in pediatric t(12;21) ETV6-RUNX1 B cell precursor acute lymphoblastic leukemia. As CBFA2T3 is a transcription regulator, we hypothesized a potential collaboration between the transcription factor RUNX1 and CBFA2T3. Here, we investigated the molecular proximity between RUNX1 and CBFA2T3 proteins on chromatin and identified a regulatory loop between RUNX1 and CBFA2T3.
 
Overall design RUNX1, CBFA2T3, H3K4me1, H3K4me3 and H3K27ac genome-wide distributions were determined using ChIP-seq. Cells used in this study are pre-B lymphoblast REH and Nalm6 cell lines and blasts from B-cell precursor acute lymphoblastic leukemia (BCP-ALL) patient. ChIP samples and libraries were done by Sérandour A.A and Debaize L. High-throughput sequencing was conducted on Illumina HiSeq 1500/2500 machines at the Human and Environmental Genomics' platform of Rennes (Biogenouest génomique, Rennes, France).
 
Contributor(s) Troadec MB, Debaize L
Citation(s) 33743795
Submission date Jul 26, 2018
Last update date Mar 22, 2021
Contact name Gilles Salbert
Organization name University Rennes 1
Department IGDR UMR6290
Street address 263 Av. Gal. Leclerc
City Rennes
ZIP/Postal code 35200
Country France
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (8)
GSM3307215 REH_H3K4me1
GSM3307216 REH_H3K4me3
GSM3307217 REH_H3K27ac
Relations
BioProject PRJNA482944
SRA SRP155331

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Supplementary file Size Download File type/resource
GSE117684_RAW.tar 3.4 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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