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Series GSE117672 Query DataSets for GSE117672
Status Public on Aug 31, 2019
Title Genome-wide single nucleotide polymorphism array analysis unveils the origin of heterozygous androgenetic complete moles
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
Summary Heterozygous CHMs have been believed as synonymous with dispermic moles (two sperm origin). The possibility of diploid sperm origin has not been considered. We assessed whether heterozygous CHMs would be of dispermic or diploid sperm origin.
In all the cases, centromeric zygosity was random, i.e., mixed status. Theoretically, if the heterozygous CHMs were of diploid sperm origin, the centromeric status will be all homozygous or all heterozygous. Thus, all the analyzed heterozygous CHMs were considered to be of dispermic origin. Three cases showed the trisomy while we did not notice them with STR analysis. Diploid sperm fertilization can be rare during the development of heterozygous androgenetic CHM.
Overall design The villous genomic DNA of the thirteen heterozygous CHMs was further analyzed using the single nucleotide polymorphism array. B allele frequency (BAF) plotting was used to determine the centromeric zygosity status of all chromosomes to discriminate dispermic origin from diploid sperm origin.
Contributor(s) Usui H, Nakabayashi K, Maehara K, Hata K, Shozu M
Citation(s) 31467376
Submission date Jul 25, 2018
Last update date Sep 03, 2019
Contact name Hirokazu Usui
Phone 81-43-222-2121
Organization name Graduate School of Medicine, Chiba University
Department Department of Reproductive Medicine
Street address 1-8-1 Inohana, Chuo-ku
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
Platforms (1)
GPL13829 Illumina HumanCytoSNP-12 v2.1 BeadChip
Samples (13)
GSM3306022 HM01
GSM3306023 HM02
GSM3306024 HM03
BioProject PRJNA482860

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Supplementary file Size Download File type/resource
GSE117672_RAW.tar 101.2 Mb (http)(custom) TAR (of TXT)
Processed data provided as supplementary file

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