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Series GSE117600 Query DataSets for GSE117600
Status Public on Dec 13, 2018
Title High-throughput in vitro DROSHA processing on 38,880 pri-miRNA variants
Organism synthetic construct
Experiment type Other
Summary To investigate the precise processing mechanism of pri-miRNA, we prepared 38,880 pri-miRNA variants which contain unique barcode sequences (Fang and Bartel, Mol Cell, 60: 131) and performed high-throughput processing experiments.
 
Overall design pri-miRNA pools which contain various sequence or structural elements were processed by recombinant DROSHA and DGCR8 proteins. Using the unique barcode information, cleavage sites per each pri-miRNA variant were identified and quantified.
 
Contributor(s) Kwon SC, Woo JS, Kim VN
Citation(s) 30554947
Submission date Jul 24, 2018
Last update date Mar 14, 2019
Contact name S. Chul Kwon
E-mail(s) kwonschul@gmail.com
Organization name Institute for Basic Science
Street address 1 Gwanak-ro
City Seoul
ZIP/Postal code 08826
Country South Korea
 
Platforms (1)
GPL19604 Illumina HiSeq 2500 (synthetic construct)
Samples (3)
GSM3304660 dictionary sequencing
GSM3304661 input sequencing
GSM3304662 cleavage fragment sequencing
Relations
BioProject PRJNA482630
SRA SRP155016

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117600_RAW.tar 688.1 Mb (http)(custom) TAR (of TXT)
GSE117600_designed_variants.txt.gz 105.1 Kb (ftp)(http) TXT
GSE117600_variant_counts.txt.gz 924.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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