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Series GSE117332 Query DataSets for GSE117332
Status Public on Aug 06, 2020
Title Targets of DNA binding, N-terminal, or C-terminal domain swapped mutant forms fo Foxa proteins in induced hepatocyte-like (iHep) cells.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We have found that three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro, and DNA binding, N-terminal, and C-terminal domains (DBD, NTD, and CTD, respectively) of Foxa proteins are swappable in the direct reprogramming of the iHep cells. Then we used ChIP-seq to explore the targets of the domain swapped mutant forms of Foxa proteins during conversion event to iHep cells.
Overall design Using anti-Foxa antibodies we performed ChIP-Seq for iHep cells which were induced by forced transduction of Hnf4alpha and domain swapped mutant forms of Foxa proteins.
Contributor(s) Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K
Citation(s) 32755593
Submission date Jul 18, 2018
Last update date Aug 10, 2020
Contact name Atsushi Suzuki
Organization name Medical Institute of Bioregulation, Kyushu University
Department Division of Organogenesis and Regeneration
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (18)
GSM3291143 iHep_4a1_NA3
GSM3291144 iHep_4a1_CA3
GSM3291145 iHep_4a2_NA3
BioProject PRJNA481781
SRA SRP154384

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Supplementary file Size Download File type/resource
GSE117332_RAW.tar 1.6 Gb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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