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| Status |
Public on Feb 11, 2019 |
| Title |
Differential gene expression in NPHS2-Cre +/+ mouse glomeruli versus wild-type control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate differential gene expression that might account for the differing glomerular phenotype of NPHS2-Cre +/+ mice when compared with wild-type control, including altered GBM thickness, loss of normal foot process morphology, and decrease in podocyte number, RNA sequencing analysis was performed on glomeruli extracted from both NPHS2-Cre +/+ and wild-type control mice.
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| Overall design |
Following isolation of glomeruli using Dynabeads from NPHS2-Cre +/+ and wild-type control mice (n=2 biological replicates per genotype, singly isolated), total RNA was extracted and RNA samples were submited for sample preparation and sequencing.
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| Contributor(s) |
Marshall CB, Balkawade RS, Crowley MR, Crossman DK |
| Citation(s) |
30810063 |
| |
| Submission date |
Jul 18, 2018 |
| Last update date |
May 13, 2019 |
| Contact name |
Caroline B Marshall |
| Organization name |
University of Alabama at Birmingham
|
| Department |
Medicine/Nephrology
|
| Street address |
1720 2nd Ave S
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA481767 |
| SRA |
SRP154372 |
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