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Series GSE117320 Query DataSets for GSE117320
Status Public on Jan 03, 2022
Title Distinct microglial transcriptional responses during opioid exposure or neuropathic pain [mouse]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In settings of heightened pain sensitivity, such as following peripheral nerve injury (PNI) or opioid-induced hyperalgesia (OIH), microglia take on an activated phenotype. Functional studies have suggested that microglia activated by PNI or chronic opioids then engage common mechanisms to facilitate pain. Here we conducted RNA sequencing of acutely isolated spinal cord microglia to comprehensively interrogate commonality between PNI and OIH. By combining our results with meta-analysis of published datasets, we identify transcriptional signatures of microglial reactivity that differ between PNI models over time, opioid exposure, or CNS pathology, despite similar histological outcomes. Collectively, these results reveal a discrepancy between histological markers of activation and transcriptional response, and provide a resource of pain-associated microglial transcriptomes that caution against a universal signature of microglia activation.
Overall design RNA sequencing of actutely isolated spinal cord microglia following peripheral nerve injury or opioid treatment, or RNA-seq of cultured rat cortical microglia after morphine treatment or TLR agonist treatment
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Contributor(s) Sypek E, Scherrer G
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Submission date Jul 18, 2018
Last update date Jan 03, 2022
Contact name Gregory Scherrer
Organization name Stanford University
Department Anesthesiology
Street address 1050 Arastradero Rd
City Palo Alto
State/province CA
ZIP/Postal code 94304
Country USA
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (30)
GSM3290974 Control 1
GSM3290975 Control 2
GSM3290976 Control 3
This SubSeries is part of SuperSeries:
GSE117321 Distinct microglial transcriptional responses during opioid exposure or neuropathic pain
BioProject PRJNA481755
SRA SRP154357

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Supplementary file Size Download File type/resource
GSE117320_Mouse_DESeq2.xlsx 13.4 Mb (ftp)(http) XLSX
GSE117320_Mouse_FPKM.txt.gz 1.6 Mb (ftp)(http) TXT
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