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Series GSE117190 Query DataSets for GSE117190
Status Public on Jul 16, 2021
Title Genome-wide DNA methylation change due to H19 knockdown and TET3 knockdown in UtLM
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary We used Methyl-MiniSeq platform from Zymo Research company to identify genome-wide methylation changes affected by H19 knockdown and TET3 knockdown in human UtLM. Following H19 and TET3 knockdown, we observed extensive genome-wide methylation pattern changes relative to siCon cells, with some genes showing increased methylation, others showing decreased methylation, and a third group with no significant change.
Overall design human UtLM were transfected with either control siRNA, siH19 or siTET3. 48h later, cellular genomic DNA was extracted and subjected to genome-scale DNA methylation mapping using the platform of an improved version of Reduced Representation Bisulfite Sequencing (RRBS).
Contributor(s) Giang K, Chung H, Sun X
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Submission date Jul 16, 2018
Last update date Jul 17, 2021
Contact name Ying Jiang
Organization name Department of Obstetrics, Gynecology & Reproductive Sciences
Street address 310 Cedar Street
City New Haven
ZIP/Postal code 06510
Country USA
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (3)
GSM3273184 siCon
GSM3273185 siH19
GSM3273186 siTET3
BioProject PRJNA481374
SRA SRP153995

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117190_RAW.tar 185.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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