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Series GSE117062 Query DataSets for GSE117062
Status Public on Jul 13, 2019
Title Histone 3 lysine 27 acetylation profiling reveals its role in the chronic DSS-induced colitis mouse model [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate whether the H3K27ac is involved in the development of inflammatory bowal disease. We established dextran sulfate sodium (DSS)-induced chronic colitis and performed RNA-sequencing and Chromatin Immunoprecipitation followed by NGS sequencing for H3K27ac in the mice colon tissues. We find that the global H3K27ac distribution in colon tissue had no significant difference, while on the typical-enhancers specific in the DSS group, H3K27ac signals were significantly enriched when compared with the control. Furthermore, we identified 89 candidate genes which represented the susceptible genes to H3K27ac change and predicted transcription factors (TFs) upon the DSS treatment. Collectively, our study revealed that H3K27ac increase on special typical-enhancers is possibly related to the development of intestinal inflammation by up-regulating adjacent gene expression and shifting TFs networks.
 
Overall design We established the dextran sulfate sodium (DSS)-induced chronic colitis and performed RNA-sequencing (RNA-seq) and Chromatin Immunoprecipitation followed by NGS sequencing (ChIP-seq) for H3K27ac in the mice colon tissues to investigate whether the H3K27ac is involved in the development of intestinal inflammation.
 
Contributor(s) Chen M, Li Q, Cao N, Deng Y, Li L, Zhao Q, Wu M, Ye M
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Submission date Jul 13, 2018
Last update date Jul 14, 2019
Contact name Qinglan Li
E-mail(s) liqinglan@whu.edu.cn
Organization name Wuhan University
Street address Room 5005, Life Science College, Wuhan University
City Wuhan City
State/province Hubei Province
ZIP/Postal code 430062
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3268046 DSS-rep1
GSM3268047 DSS-rep2
GSM3268048 DSS-rep3
Relations
BioProject PRJNA480961
SRA SRP153337

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117062_RAW.tar 910.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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