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Series GSE116915 Query DataSets for GSE116915
Status Public on Oct 01, 2018
Title RNAseq quantitative analysis of ion channel mRNA expression in microbead occlusion model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of this study was to utilize RNAseq to determine if there are changes in ion channel mRNA expression in the retina of C57BL/6 mice subjected to the Microbead Occlusion Model. Whole retina was isolated from 2 month old mice that received intracameral injections of either saline or 15-um-diameter microbeads. Total RNA was isolated from the retinas and subjected to RNA sequencing using Illumina HiSeq 2500. Fold change in mRNA expression was compared between saline- and microbead-injected samples. We then analyzed these data for changes in expression of potassium channels, such as tandem-pore domain K+ channels, inwardly rectifying K+ channels, and the Na+/K+-ATPase.
Overall design Retinal mRNA profiles of saline- and microbead-injected C57BL/6 mice were generated by whole genome sequencing using Illumina NovaSeq6000
Contributor(s) Sappington RM, Fischer RA
Citation(s) 31736686
Submission date Jul 11, 2018
Last update date Nov 25, 2019
Contact name Lauren Katie Wareham
Phone 8575408019
Organization name Vanderbilt University Medical Center
Department Vanderbilt Eye Institute
Lab Sappington Lab
Street address 2213 Garland Avenue, MRBIV 11435a
City Nashville
State/province Tennessee
ZIP/Postal code 37232
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM3263639 C597 Tag#984
GSM3263640 C598 Tag#985
GSM3263641 C600 Tag#987
BioProject PRJNA480596
SRA SRP153029

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Supplementary file Size Download File type/resource
GSE116915_Microbead_vs_control_raw_data_RNAseq_mouse.define_DESeq2.txt.gz 1.2 Mb (ftp)(http) TXT
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