|
Status |
Public on Oct 01, 2018 |
Title |
RNAseq quantitative analysis of ion channel mRNA expression in microbead occlusion model |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
The purpose of this study was to utilize RNAseq to determine if there are changes in ion channel mRNA expression in the retina of C57BL/6 mice subjected to the Microbead Occlusion Model. Whole retina was isolated from 2 month old mice that received intracameral injections of either saline or 15-um-diameter microbeads. Total RNA was isolated from the retinas and subjected to RNA sequencing using Illumina HiSeq 2500. Fold change in mRNA expression was compared between saline- and microbead-injected samples. We then analyzed these data for changes in expression of potassium channels, such as tandem-pore domain K+ channels, inwardly rectifying K+ channels, and the Na+/K+-ATPase.
|
|
|
Overall design |
Retinal mRNA profiles of saline- and microbead-injected C57BL/6 mice were generated by whole genome sequencing using Illumina NovaSeq6000
|
|
|
Contributor(s) |
Sappington RM, Fischer RA |
Citation(s) |
31736686 |
|
Submission date |
Jul 11, 2018 |
Last update date |
Nov 25, 2019 |
Contact name |
Lauren Katie Wareham |
E-mail(s) |
lauren.wareham@vanderbilt.edu
|
Phone |
8575408019
|
Organization name |
Vanderbilt University Medical Center
|
Department |
Vanderbilt Eye Institute
|
Lab |
Sappington Lab
|
Street address |
2213 Garland Avenue, MRBIV 11435a
|
City |
Nashville |
State/province |
Tennessee |
ZIP/Postal code |
37232 |
Country |
USA |
|
|
Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA480596 |
SRA |
SRP153029 |