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Status |
Public on Jan 15, 2019 |
Title |
Contribution of SRF and Nkx2-5 to androgen-dependent gene expression in prostate cancer |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To study and compare the effect of siRNA-mediated SRF or Nkx2-5 silencing on androgen-responsive genes in LNCaP cells.
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Overall design |
Human LNCaP prostate cancer cells were transfected with siRNA SmartPools targeting SRF or Nkx2-5 or a non-targeting siRNA SmartPool. Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (5nM) or vehicle. Three biological replicates were generated per treatment group. Forty-eight hours later, total RNA was isolated and processed for RNA-Seq analysis.
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Contributor(s) |
Ben-Salem S, Senapati D, Balaji Venkadakrishnan V, Su Y, Cortes E, Hu Q, Liu S, Heemers H |
Citation missing |
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Submission date |
Jul 06, 2018 |
Last update date |
Mar 27, 2019 |
Contact name |
Eduardo Cortes |
E-mail(s) |
eduardo.cortesgomez@roswellpark.org
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Organization name |
ROSWELL PARK CANCER INSTITUTE
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Department |
Biostatistics and Bioinformatics
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Street address |
ELM AND CARLTON STREETS
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City |
BUFFALO |
State/province |
NY |
ZIP/Postal code |
14263 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (18)
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Relations |
BioProject |
PRJNA480000 |
SRA |
SRP152523 |