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Series GSE116728 Query DataSets for GSE116728
Status Public on Jan 15, 2019
Title Contribution of SRF and Nkx2-5 to androgen-dependent gene expression in prostate cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To study and compare the effect of siRNA-mediated SRF or Nkx2-5 silencing on androgen-responsive genes in LNCaP cells.
 
Overall design Human LNCaP prostate cancer cells were transfected with siRNA SmartPools targeting SRF or Nkx2-5 or a non-targeting siRNA SmartPool. Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (5nM) or vehicle. Three biological replicates were generated per treatment group. Forty-eight hours later, total RNA was isolated and processed for RNA-Seq analysis.
 
Contributor(s) Ben-Salem S, Senapati D, Balaji Venkadakrishnan V, Su Y, Cortes E, Hu Q, Liu S, Heemers H
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Submission date Jul 06, 2018
Last update date Mar 27, 2019
Contact name Eduardo Cortes
E-mail(s) eduardo.cortesgomez@roswellpark.org
Organization name ROSWELL PARK CANCER INSTITUTE
Department Biostatistics and Bioinformatics
Street address ELM AND CARLTON STREETS
City BUFFALO
State/province NY
ZIP/Postal code 14263
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (18)
GSM3259535 control siRNA vehicle 1
GSM3259536 control siRNA vehicle 2
GSM3259537 control siRNA vehicle 3
Relations
BioProject PRJNA480000
SRA SRP152523

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Supplementary file Size Download File type/resource
GSE116728_RawCounts.csv.gz 1.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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