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Series GSE116657 Query DataSets for GSE116657
Status Public on Jun 30, 2019
Title Leo1 is essential for dynamic regulation of heterochromatin and gene expression during cellular quiescence [RNA-Seq]
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by high throughput sequencing
Summary Cellular quiescence is a reversible differentiation state when cells are changing the gene expression programme to reduce metabolic functions and adapt to a new cellular environment. The epigenetic changes that accompany these alterations are not so well understood. Here we investigate the role of Leo1, a subunit of the conserved Paf1 (RNA polymerase-associated factor 1) complex, in the quiescence process using fission yeast as a model organism. Fission yeast cells enter the G0 phase of the cell cycle when exposed to nitrogen starvation and the heterochromatin regions become very dynamic. The reduction of heterochromatin in early G0 correlates with the reduced activity of target of rapamycin, TORC2, signalling. Cells lacking the Leo1 show reduced survival in G0. In these cells heterochromatin regions including subtelomeres are stabilized and many genes, including membrane transport genes, fail to be expressed. Our results suggest that Leo1 is essential for dynamic regulation of heterochromatin and gene expression during cellular quiescence.
Overall design RNA profiles in wt/leo1D during quiescence; total 20 samples; biological duplicates
Contributor(s) Oya E, Dubief MD, Cohen A, Schurra C, Nakayama J, Weisman R, Arcangioli B, Ekwall K
Citation(s) 31315658
Submission date Jul 05, 2018
Last update date Sep 29, 2019
Contact name Eriko Oya
Organization name Karolinska Institutet
Street address Blickagången 16
City Huddinge
ZIP/Postal code 141 83
Country Sweden
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (20)
GSM3244910 wt_T0_rep1
GSM3244911 wt_T8_rep1
GSM3244912 wt_T24_rep1
BioProject PRJNA479830
SRA SRP152284

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE116657_DiffExpreAnalysis_cpm1_3.xlsx 1.8 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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