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Series GSE116603 Query DataSets for GSE116603
Status Public on Feb 28, 2019
Title Dissecting the Role of H3K27 Acetylation and Methylation in PRC2 Mediated Control of Cellular Identity
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The Polycomb repressive complexes PRC1 and PRC2 act non-redundantly at target genes to maintain transcriptional programs and ensure cellular identity. PRC2 methylates lysine 27 on histone H3 (H3K27me), while PRC1 mono-ubiquitinates histone H2A at lysine 119 (H2Aub1). Here we present engineered mouse embryonic stem cells (ESCs) targeting the PRC2 subunits EZH1 and EZH2 to discriminate between contributions of distinct H3K27 methylation states and the presence of PRC2/1 at chromatin. We generate catalytically inactive EZH2 mutant ESCs, demonstrating that H3K27 methylation, but not recruitment to the chromatin, is essential for proper ESC differentiation. We further show that EZH1 activity is sufficient to maintain repression of Polycomb targets by depositing H3K27me2/3 and preserving PRC1 recruitment. This occurs in the presence of altered H3K27me1 deposition at actively transcribed genes and by a diffused hyperacetylation of chromatin that compromises ESC developmental potential. Overall, this work provides insights for the contribution of diffuse chromatin invasion by acetyltransferases in PRC2-dependent loss of developmental control.
Overall design Evaluation of SUZ12 and RING1B chromatin binding and of H3K27me1, H3K27me2, H3K27me3, H3k27ac and H2AK119ub deposition in different CRISPR/Cas9-edited mouse embryonic stem cells.
Contributor(s) Lavarone E, Barbieri CM, Pasini D
Citation(s) 30976011
Submission date Jul 03, 2018
Last update date Apr 23, 2019
Contact name Diego Pasini
Organization name European Institute of Oncology
Street address Via Adamello, 16
City Milano
ZIP/Postal code 20139
Country Italy
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (50)
GSM3243624 WT_SUZ12_ChipSeq
GSM3243625 Ezh2KO_SUZ12_ChipSeq
GSM3243626 Ezh1KO_Ezh2KO_SUZ12_ChipSeq
BioProject PRJNA479477
SRA SRP151926

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Supplementary file Size Download File type/resource
GSE116603_RAW.tar 144.5 Mb (http)(custom) TAR (of GAPPEDPEAK, NARROWPEAK, TXT)
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Processed data provided as supplementary file
Raw data are available in SRA

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