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Status |
Public on Dec 21, 2018 |
Title |
Identification and validation of a tumor-infiltrating Treg transcriptional signature conserved across species and tumor types. |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We reported transcriptional characterization of tumor-infiltrating T regulatory cells (TITRs) that is shared between species and among different tumor types and stages. We genomically profiled immunocytes from 2 species: 1. Mouse: CD4+ Treg and Tconv, and CD8+ T cells from tumor and spleen of B16, MC38 or CT26 tumor bearing mice, as well as from spleens of non-tumor bearing mice. 2. Human: CD4+ Treg cells from surgically resected and cryopreserved human colorectal carcinomas or normal colon tissue.
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Overall design |
Gene expression profiles of CD4+ Treg and Tconv, and CD8+ T cells from tumor and spleen of B16, MC38 or CT26 tumor bearing mice (biological triplicates), as well as from spleens of non-tumor bearing mice, and CD4+ Treg cells from 12 surgically resected and cryopreserved human colorectal carcinomas or normal colon tissue.
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Contributor(s) |
Magnuson A, Benoist C, Mathis D |
Citation(s) |
30348759 |
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Submission date |
Jun 27, 2018 |
Last update date |
Mar 26, 2019 |
Contact name |
CBDM Lab |
E-mail(s) |
cbdm@hms.harvard.edu
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Phone |
617-432-7747
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Organization name |
Harvard Medical School
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Department |
Microbiology and Immunobiology
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Lab |
CBDM
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Street address |
77 Avenue Louis Pasteur
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
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Platforms (2) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (78)
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Relations |
BioProject |
PRJNA478226 |
SRA |
SRP151471 |