Expression profiling by high throughput sequencing
Cardiac-specific Bag3-P209L transgenic (Tg+) mice develop dilated cardiomyopathy by 12 months of age. The goals of this project are to utilize RNA-sequencing to compare both cardiomyocyte and cardiac fibroblast transcriptomes between aged Bag3-P209L Tg+ and Bag3-WT mice to identify cardiomyocyte and fibroblast specific gene sets that contribute to the functional and morphological changes previously identified in Bag3-P209L Tg+ hearts.
Both cardiomyocytes and fibroblasts were isolated from three 16-month old Bag3-P209L Tg+ and three age matched Bag3-WT mice. RNA from cardiomyocytes and fibroblasts were then extracted and submitted to Novogene for cDNA library creation and subsequent RNA sequencing.