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| Status |
Public on Nov 22, 2018 |
| Title |
An Optically Decodable Bead Array for Linking Imaging and Sequencing with Single-Cell Resolution |
| Organisms |
Homo sapiens; Mus musculus; synthetic construct |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Optically decodable beads link the identity of an analyte or sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We have combined microfluidics with optically decodable beads to link phenotypic analysis of living cells to sequencing. As a proof-of-concept, we applied this to demonstrate an accurate and scalable tool for connecting live cell imaging to single-cell RNA-Seq called Single Cell Optical Phenotyping and Expression (SCOPE-Seq).
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| |
|
| Overall design |
Performed SCOPE-Seq on thousands of cells from two cell lines.
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| |
|
| Contributor(s) |
Yuan J, Sims PA |
| Citation(s) |
30583733 |
| Submission date |
Jun 19, 2018 |
| Last update date |
Feb 27, 2019 |
| Contact name |
Peter Sims |
| Organization name |
Columbia University
|
| Street address |
3960 Broadway, 2nd Floor, Room 203AC
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
| GPL19424 |
Illumina NextSeq 500 (synthetic construct) |
|
| Samples (11)
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|
| Relations |
| BioProject |
PRJNA476792 |
| SRA |
SRP150876 |
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