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| Status |
Public on Jan 14, 2020 |
| Title |
Impact of HSF1 and HSF2 on gene expression in normal growth conditions and upon proteotoxic stress. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA sequencing (RNA-seq) was used to study the impact of heat shock factors 1 and 2 (HSF1 and HSF2) on gene expression in normal growth conditions, upon Bortezomib-induced proteasome inhibition (25 nM, 6 h or 10 h), and upon heat shock (42∘C, 1 h).
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| Overall design |
U2OS WT, HSF1 KO, and HSF2 KO were treated with 25 nM of Bortezomib for 6 h or 10 h, or heat shocked at 42 degrees for 1 h. Samples were prepared in biological quadruplets.
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| Contributor(s) |
Joutsen J, Da Silva AJ, Luoto JC, Budzynski MA, Nylund AS, de Thonel A, Concordet J, Mezger V, Sabéran-Djoneidi D, Henriksson E, Sistonen L |
| Citation(s) |
31940498 |
| Submission date |
Jun 18, 2018 |
| Last update date |
Apr 23, 2020 |
| Contact name |
Jenny Maaria Joutsen |
| E-mail(s) |
jenny.joutsen@abo.fi
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| Organization name |
Åbo Akademi University
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| Department |
Cell Biology
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| Lab |
Lea Sistonen
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| Street address |
Tykistökatu 6A
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| City |
Turku |
| State/province |
Varsinais-Suomi |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA476608 |
| SRA |
SRP150792 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE115973_NormalizedData_withAnnotations.xls.gz |
8.5 Mb |
(ftp)(http) |
XLS |
| GSE115973_RAW.tar |
322.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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