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| Status |
Public on Apr 25, 2019 |
| Title |
Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Intestinal organoids are complex three-dimensional structures that mimic cell type composition and tissue organization of the intestine by recapitulating the self-organizing capacity of cell populations derived from a single stem cell. Crucial in this process is a first symmetry-breaking event, in which only a fraction of identical cells in a symmetrical cyst differentiate into Paneth cells, which in turn generates the stem cell niche and leads to asymmetric structures such as crypts and villi. We here combine a quantitative single-cell gene expression and imaging approach to characterize the development of intestinal organoids from a single cell. We show that intestinal organoid development follows a regeneration process driven by transient Yap1 activation. Cell-to-cell variability in Yap1, emerging in symmetrical cysts, initiates a Notch/Dll1 lateral inhibition event driving the symmetry-breaking event and the formation of the first Paneth cell. Our findings reveal how single cells exposed to a uniform growth-promoting environment have the intrinsic ability to generate emergent, self-organized behavior resulting in the formation of complex multicellular asymmetric structures.
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| |
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| Overall design |
Intestinal organoids mRNA profiles for each day of organoid development (day 0 – day 5) in triplicate. Three different organoid lines C57BL/6 wild type have been used.
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| Contributor(s) |
Serra D, Mayr U, Boni A, Lukonin I, Rempfler M, Challet Meylan L, Stadler MB, Strnad P, Papasaikas P, Vischi D, Waldt A, Roma G, Liberali P |
| Citation(s) |
31019299 |
| |
| Submission date |
Jun 18, 2018 |
| Last update date |
May 26, 2019 |
| Contact name |
Denise Serra |
| E-mail(s) |
dns.serra@gmail.com
|
| Organization name |
FMI
|
| Street address |
Maulbeerstrasse 14
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE115958 |
Self-organization and symmetry breaking in intestinal organoid development |
|
| Relations |
| BioProject |
PRJNA476589 |
| SRA |
SRP150776 |
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