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Series GSE115950 Query DataSets for GSE115950
Status Public on Sep 25, 2018
Title Neonatally imprinted mesenteric lymph node stromal cell subsets induce tolerogenic dendritic cells [migDC]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Gut-draining mesenteric lymph nodes (mLNs) play a key role in peripheral tolerance towards food and commensal antigens by providing an optimal microenvironment for efficient de novo induction of Foxp3+ regulatory T cells (Tregs). We recently identified mLN stromal cells as critical cellular players in this process and demonstrated that their tolerogenic properties are imprinted by microbiota. Here, we show that this imprinting process already takes place in the neonatal phase and renders the mLN stromal cell compartment resistant to inflammatory perturbations later in life. Utilizing LN transplantation, RNA-seq and single-cell RNA-seq allowed identification of stably imprinted expression signatures in mLN fibroblastic stromal cells. We dissected common stromal cell subsets across gut-draining mLNs and skin-draining LNs with location-specific immunomodulatory functions, such as subset-specific expression of Aldh1a2/3. Accordingly, mLN stromal cells shaped resident dendritic cells to attain high Treg-inducing capacity in a Bmp2-dependent manner. Thus, crosstalk between mLN stromal and resident dendritic cells provides a robust feedback mechanism for the maintenance of intestinal tolerance.
Overall design Transcriptomic analysis of migratory dendritic cells of skin-draining and intestinal-draining lymph nodes from endogenous and lymph nodes transplanted to the popliteal fossa.
Contributor(s) Pezoldt J, Pasztoi M, Zou M, Wiechers C, Beckstette M, Thierry G, Vafadarnejad E, Floess S, Arampatzi P, Buettner M, Schweer J, Fleissner D, Vital M, Pieper DH, Basic M, Dersch P, Strowig T, Hornef M, Bleich A, Bode U, Pabst O, Bajenoff M, Saliba A, Huehn J
Citation(s) 30254319, 36227687
Submission date Jun 18, 2018
Last update date Jan 05, 2023
Contact name Joern Pezoldt
Phone 0041766040171
Organization name EPFL
Department SV
Lab Laboratory Systems Biology and Genetics
Street address Station 19, SV 3818.A
City Lausanne
ZIP/Postal code 1015
Country Switzerland
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (20)
GSM3195907 pLN_SPF_Tx_migDC_1
GSM3195908 pLN_SPF_Tx_migDC_2
GSM3195909 pLN_SPF_Tx_migDC_3
This SubSeries is part of SuperSeries:
GSE116633 Neonatally imprinted mesenteric lymph node stromal cell subsets induce tolerogenic dendritic cells
BioProject PRJNA476578
SRA SRP150769

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Supplementary file Size Download File type/resource
GSE115950_DESeq2_genes_diffexp_by_fc_Migr_mLN_SPF_Tx_vs_Migr_pLN_SPF_Tx.csv.gz 1.5 Mb (ftp)(http) CSV
GSE115950_DESeq2_genes_diffexp_by_fc_Migr_mLN_SPF_vs_Migr_pLN_SPF.csv.gz 1.4 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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