GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE115865 Query DataSets for GSE115865
Status Public on Jun 23, 2018
Title Neurotropism and Neurotoxicity Comparison among Retrograde Viral Tracers and Their Combinational Application in High-order Circuit Tracing
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here, we demonstrated the neurotropism of different retrograde viral tracers are distinct through parallel comparison of multi-synaptic and mono-synaptic tracers in circuit tracing. By virture of their distinct tropism, we reconstructed more comprehensive LHA input circuit map. Notably, exogenous expression of TVA receptor in rabies virus (RV) resistant neurons can enable the infection of EnVA-pseudotyped RV. The potential viral receptor candidates attributing to neurotropism were analyzed by single cell sequencing. Further, we systematically compared neurotoxicity of glycoprotein-deleted RV and rAAV2-retro by RNAseq and immunohistochemistry. Finally, we demonstrated a proof-of-concept strategy for high-order circuit tracing by combining different viral tracers.
Overall design Neurotropism and neurotoxicity comparison of different retrograde viral tracers and their combinational application in high-order circuit tracing
Contributor(s) Li W, Xu W, Zou Y
Citation(s) 30736827
Submission date Jun 15, 2018
Last update date Mar 01, 2019
Contact name Gang Cao
Organization name Huazhong Agricultural University
Lab State Key Laboratory of Agricultural Microbiology
Street address Lion Rock One
City wuhan
State/province hubei
ZIP/Postal code 430070
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (73)
GSM3192097 rAAV2_mPFC_L2
GSM3192098 rAAV1_mPFC_L2
GSM3192099 RV1_LHA_L2
BioProject PRJNA476331
SRA SRP150630

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE115865_RNAseq_expression.xls.gz 1.5 Mb (ftp)(http) XLS
GSE115865_single_cell_expression.xls.gz 3.2 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap