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| Status |
Public on Jan 07, 2009 |
| Title |
FAIRE-chip in MCF7 and LNCaP |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
The compaction degree of chromatin is intimately related to its functionality and active cis-regulatory elements typically exist within open chromatin regions depleted in nucleosomes (Heintzman et al. 2007; Boyle et al. 2008). These domains can be identified using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) that allows for enrichment of nucleosome-depleted genomic regions when cross-linked chromatin is subjected to phenol-chloroform extraction (Nagy et al. 2003; Hogan et al. 2006)
Here, chromatin structure was analyzed in MCF7 and LNCaP cells using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) (Nagy et al. 2003; Hogan et al. 2006)
Keywords: FAIRE-chip
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| Overall design |
MCF7 and LNCaP cells were grown in full media, cross-linked and processed as in (Nagy et al. 2003; Hogan et al. 2006). DNA obtained by FAIRE was hybridized to Human Affymetrix tiled array F. Triplicates of FAIRE and input DNA are provided (4 inputs for MCF7 cells).
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| |
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| Contributor(s) |
Eeckhoute J |
| Citation(s) |
19129543 |
| |
| Submission date |
May 28, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Jérôme Eeckhoute |
| E-mail(s) |
jerome.eeckhoute@inserm.fr
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| Organization name |
CNRS
|
| Lab |
INSERM UMR 1011, Université Lille-Nord de France
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| Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
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| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
| |
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| Platforms (1) |
| GPL4915 |
[Hs35b_P06R] Affymetrix Human Tiling 2.0R Set, Array 6 |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA106209 |
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