|
| Status |
Public on Jan 24, 2020 |
| Title |
RNA sequencing on circulating tumor cells from prostate cancer patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Circulating tumor cells (CTCs) undergo considerable stress in the bloodstream. To understand the underlying phenomenon, we conducted single-cell genomic or transcriptomic analyses of primary tumor cells shed in urine and CTCs in blood of prostate cancer patients. Of more than four hundred focal genomic alterations analyzed, increased and decreased copy-numbers of 113 recurrent regions were found in CTCs relative to primary tumor cells. Tumor cells with these pre-existing genomic alterations can be adaptively selected, allowing for transcription reprogramming during blood circulation. These regions harbored genes associated with oxidative phosphorylation machineries that are exploited by CTCs for alternative energy metabolism.
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| |
|
| Overall design |
Examination of prostate CTCs transcriptomic profile in single cell level
|
| |
|
| Contributor(s) |
Liss MA |
| Citation(s) |
31805710 |
| Submission date |
Jun 08, 2018 |
| Last update date |
Jan 24, 2020 |
| Contact name |
Chun-Lin Lin |
| E-mail(s) |
linc3@uthscsa.edu
|
| Phone |
2104508471
|
| Organization name |
University of Texas Health Science Center at San Antonio
|
| Street address |
7703 Floyd Curl Dr., MC8257, 233 STRF
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (136)
|
|
| Relations |
| BioProject |
PRJNA475218 |
| SRA |
SRP150061 |
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