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Series GSE115377 Query DataSets for GSE115377
Status Public on Mar 12, 2019
Title Displacement of WDR5 from chromatin by a pharmacological WIN site inhibitor with picomolar affinity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We discovered potent small molecule inhibitors against the WIN site of WDR5. These inhibitors selectively block the proliferation of mammalian cells carrying fusions of the MLL1 oncogene. Here, we show that these inhibitors result in the rapid displacement of WDR5 from chromatin in both sensitive (MV4:11) and non-sensitive (K562) cell lines, induce early changes in the distribution of active polymerases at a subset of WDR5-bound genes, and induce global transcript changes that are consistent with induction of the tumor suppressor p53.
Overall design MV4:11 or K562 cells were treated with one of two WIN site inhibitors (C3 or C6), a negative control compound (C6nc), or DMSO. ChIP-Seq was used to track interaction of WDR5 with chromatin; PRO-Seq was used to track early changes in the distribution of active RNA polymerases, and RNA-Seq was used to monitor long-term transcriptional changes. RNA-Seq was also performed on MV4:11 cells treated with the HDM2 inhibitor nultin.
Contributor(s) Tansey WP, Aho ER, Howard GC, Liu Q, Wang J, Acharya P
Citation(s) 30865883, 31996893
Submission date Jun 05, 2018
Last update date Jun 09, 2020
Contact name Jing Wang
Organization name Vanderbilt University Medical Center
Street address 2220 Pierce Avenue
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (77)
GSM3176959 K562 ChIP-seq DMSO IgG rep1
GSM3176960 K562 ChIP-seq DMSO IgG rep2
GSM3176961 K562 ChIP-seq C6nc IgG rep1
BioProject PRJNA474736
SRA SRP149838

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE115377_K562-MV411_RNA-seq.counts.txt.gz 2.6 Mb (ftp)(http) TXT
GSE115377_K562-N1_566_peaks.narrowPeak.gz 15.6 Kb (ftp)(http) NARROWPEAK
GSE115377_K562-N1_641_peaks.narrowPeak.gz 13.6 Kb (ftp)(http) NARROWPEAK
GSE115377_K562-N1_DM_peaks.narrowPeak.gz 31.7 Kb (ftp)(http) NARROWPEAK
GSE115377_K562-N2_566_peaks.narrowPeak.gz 28.9 Kb (ftp)(http) NARROWPEAK
GSE115377_K562-N2_641_peaks.narrowPeak.gz 21.6 Kb (ftp)(http) NARROWPEAK
GSE115377_K562-N2_DM_peaks.narrowPeak.gz 18.6 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N1_566_D9E1I_peaks.narrowPeak.gz 6.3 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N1_641_D9E1I_peaks.narrowPeak.gz 2.6 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N1_DM_429A_peaks.narrowPeak.gz 9.6 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N1_DM_D9E1I_peaks.narrowPeak.gz 5.8 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N2_566_D9E1I_peaks.narrowPeak.gz 6.9 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N2_641_D9E1I_peaks.narrowPeak.gz 675 b (ftp)(http) NARROWPEAK
GSE115377_MV411-N2_DM_429A_peaks.narrowPeak.gz 4.0 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N2_DM_D9E1I_peaks.narrowPeak.gz 7.2 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N3_566_D9E1I_peaks.narrowPeak.gz 2.8 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N3_641_D9E1I_peaks.narrowPeak.gz 1.3 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N3_DM_429A_peaks.narrowPeak.gz 3.0 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411-N3_DM_D9E1I_peaks.narrowPeak.gz 7.2 Kb (ftp)(http) NARROWPEAK
GSE115377_MV411_DMSO-Nutlin_RNA-seq.counts.txt.gz 972.2 Kb (ftp)(http) TXT
GSE115377_PROseq-pp_gb_counts.txt.gz 1.5 Mb (ftp)(http) TXT
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