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| Status |
Public on Jul 17, 2020 |
| Title |
Ongoing classical IL-6 signaling is required to maintain a pathogenic Th17 response in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Th17 precursors from WT and IL6ra-/- mice were co-transferred into Rag1-/- mice to induce colitis. RNA was isolated from CD4+ T cells pre-transfer and from colonic CD4+ T cells 4 weeks post- transfer
Another aim is to compare gene expression of WT (IL-6) v WT (HDS) v IL6RaKO (HDS) in vitro polarized Thy1.1+ Th17p cells
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| Overall design |
WT (CD45.1) and Il6ra-/- (CD45.2) IL-17F/Thy1.1 CD4 T cells were cultured under Th17 conditions in vitro (2.5 ug/ml anti-CD3, 10 ug/ml anti-IFN-g, 10 ug/ml anti-IL-4, 2.5 ng/ml TGF-b, 20 ng/ml hyper-IL-6, with irradiated splenic APCs) then Th17p (Thy1.1+) were harvested (pre_WT and pre_KO) and co-transferred into Rag-/- mice to induce colitis. Four weeks after transfer, live CD4+ TCRb+ cells from the colon of recipient mice were sorted into CD45.1+ WT (post_WT) and CD45.2+ IL-6Ra KO (post_KO) groups for RNA-Seq analysis.
3 samples (WT IL-6, WT HDS63, KO HDS63) of T cells from spleen and lymph nodes with 2 replicates each (6 samples), plus two controls (WT Tn and KO Tn) also with two replicates (4 samples).
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| Contributor(s) |
Harbour S, Gao M, Witte S, Hatton R, Weaver C |
| Citation(s) |
32680955 |
| |
| Submission date |
May 31, 2018 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Min Gao |
| E-mail(s) |
mgao@uabmc.edu
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| Organization name |
University of Alabama at Birmingham
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| Street address |
1900 University Blvd, THT Building Suite #130G
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| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA473914 |
| SRA |
SRP149419 |
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