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Series GSE115112 Query DataSets for GSE115112
Status Public on Jun 15, 2018
Title Estrogen responsive transcriptome of estrogen receptor positive normal human breast cells in 3D cultures
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Understanding how differentiation, microenvironment, and hormonal milieu influence human breast cell susceptibility to malignant transformation will require the use of physiologically relevant in vitro systems. We developed a 3D culture model that enables the propagation of normal estrogen receptor alpha (ER)+ cells. The purpose of this experiment was to assess ER functionality and compare estrogen-induced transcripts among samples and systems.
Overall design RNA-seq was performed on RNA prepared from replicate 3D cultures from 3 normal 3D breast culture specimens exposed to 10nM estradiol or vehicle alone for 6 or 24 hours.
Contributor(s) Yaswen P, Loguinov A
Citation(s) 30993572
Submission date May 30, 2018
Last update date Apr 21, 2019
Contact name Paul Yaswen
Phone 5107346880
Organization name LBNL
Street address One Cyclotron Road, Building 977
City Berkeley
State/province California
ZIP/Postal code 94720
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (39)
GSM3165452 N182-C1
GSM3165453 N182-C2
GSM3165454 N182-C3
BioProject PRJNA473820
SRA SRP149366

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Supplementary file Size Download File type/resource
GSE115112_UCSC_Human_genome.gtf.gz 14.3 Mb (ftp)(http) GTF
GSE115112_UCSC_Main_on_Human_kgXref.csv.gz 5.0 Mb (ftp)(http) CSV
GSE115112_coldata.csv.gz 1.6 Kb (ftp)(http) CSV
GSE115112_normalized_counts_filtered.csv.gz 6.9 Mb (ftp)(http) CSV
GSE115112_raw_counts_filtered.csv.gz 1.7 Mb (ftp)(http) CSV
GSE115112_raw_counts_unfiltered.csv.gz 2.2 Mb (ftp)(http) CSV
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Processed data are available on Series record

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