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| Status |
Public on Jun 15, 2018 |
| Title |
Estrogen responsive transcriptome of estrogen receptor positive normal human breast cells in 3D cultures |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding how differentiation, microenvironment, and hormonal milieu influence human breast cell susceptibility to malignant transformation will require the use of physiologically relevant in vitro systems. We developed a 3D culture model that enables the propagation of normal estrogen receptor alpha (ER)+ cells. The purpose of this experiment was to assess ER functionality and compare estrogen-induced transcripts among samples and systems.
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| |
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| Overall design |
RNA-seq was performed on RNA prepared from replicate 3D cultures from 3 normal 3D breast culture specimens exposed to 10nM estradiol or vehicle alone for 6 or 24 hours.
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| |
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| Contributor(s) |
Yaswen P, Loguinov A |
| Citation(s) |
30993572 |
| Submission date |
May 30, 2018 |
| Last update date |
Apr 21, 2019 |
| Contact name |
Paul Yaswen |
| E-mail(s) |
P_Yaswen@lbl.gov
|
| Phone |
5107346880
|
| Organization name |
LBNL
|
| Street address |
One Cyclotron Road, Building 977
|
| City |
Berkeley |
| State/province |
California |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (39)
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| Relations |
| BioProject |
PRJNA473820 |
| SRA |
SRP149366 |
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