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Series GSE115044 Query DataSets for GSE115044
Status Public on Oct 23, 2019
Title Massively parallel characterization of regulatory dynamics during neural induction [ATAC-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The molecular components governing neural induction remain largely unknown. Here, we used a suite of genomic and computational tools to comprehensively identify these components. We performed RNA-seq, ChIP-seq (H3K27ac, H3K27me3) and ATAC-seq on human embryonic stem cells (hESCs) at seven early neural differentiation time points (0-72 hours) and identified thousands of induced genes and their putative regulatory regions. We analyzed the function of ~2,500 selected regions using massively parallel reporter assays at all time points. We found numerous temporal enhancers that correlated with similarly timed epigenetic marks and gene expression. Development of a prioritization method that incorporated all genomic data identified key transcription factors (TFs) involved in neural induction. Individual overexpression of eleven TFs and several combinations in hESCs found novel neural induction regulators. Combined, our results provide a comprehensive map of genes and functional regulatory elements involved in neural induction and identify master regulator TFs that are instrumental for this process.
 
Overall design RNA-seq (3 replicates), ChIP-seq (H3K27ac, H3K27me3 - 2 replicates) and ATAC-seq (2 replicates) were preformed on human embryonic stem cells (hESCs) at seven early neural differentiation time points (0-72 hours). Additionally, the function of ~2,500 selected regions was analyzed using massively parallel reporter assays at all time points (3 replicates).
 
Contributor(s) Inoue F, Kreimer A, Ahituv N, Yosef N
Citation(s) 31631012, 38296821
Submission date May 29, 2018
Last update date Mar 20, 2024
Contact name Anat Kreimer
E-mail(s) anat.kreimer@berkeley.edu
Organization name University of California, Berkeley
Street address 378 Stanley Hall
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (20)
GSM3163868 ATACseq_3TP_0hr_rep1
GSM3163869 ATACseq_3TP_3hr_rep1
GSM3163870 ATACseq_3TP_72hr_rep1
This SubSeries is part of SuperSeries:
GSE115046 Massively parallel characterization of regulatory dynamics during neural induction
Relations
BioProject PRJNA473882
SRA SRP149492

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Supplementary file Size Download File type/resource
GSE115044_RAW.tar 19.8 Mb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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