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| Status |
Public on May 30, 2018 |
| Title |
Single-cell RNA-seq analysis of human tonsil dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed single-cell RNA-seq on dendritic cells isolated from the tonsils of one healthy donor. We used the 10x chromium technology.The expression matrix provided in supplementary is the result of Cellranger aggregate method on : GSE115007, GSE115006 and GSE103544.
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| Overall design |
Tonsil phagocytes were prepared by centrifugation on a Ficoll gradient. Dendritic cells were enriched by negative selection using magnetic beads. Cell populations were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer’s protocol.
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| Contributor(s) |
Segura E, Durand M, Bohec M, Gueguen P |
| Citation(s) |
29967419 |
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| Submission date |
May 29, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Paul Gueguen |
| E-mail(s) |
paul.gueguen@unil.ch
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| Organization name |
Institut Curie
|
| Department |
Inserm U932
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| Street address |
26 rue D'Ulm
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| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA473509 |
| SRA |
SRP149160 |
