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Series GSE114982 Query DataSets for GSE114982
Status Public on Jul 16, 2019
Title Long-term persistent infection with equine arteritis virus is associated with the upregulation of specific CD8+ T lymphocyte transcription factors, inhibitory receptors, and the CXCL16/CXCR6 axis in the ampullae of the stallion reproductive tract
Organism Equus caballus
Experiment type Expression profiling by high throughput sequencing
Summary We performed whole transcriptome analysis of the ampullae, the primary site of EAV persistence, derived from long-term carrier stallions to understand the molecular signature of persistent infection. We demonstrated that long-term persistence is characterized by a local CD8+ T lymphocyte response predominantly driven by the transcription factors EOMES and NFATC2.We showed that EAV persistence is associated with an enhanced expression of CXCL16/CXCR6 in infiltrating lymphocytes. Furthermore, we established a link between the CXCL16 genotype and the gene expression profile at the site of persistence following EAV infection. Co-expression network analysis identified CXCL16 as a “hub” gene, likely driving a specific transcriptional network.
 
Overall design We have perfomed whole transcriptome sequencing (RNAseq) on 12 ampulla samples derived from naïve stallions (n=3), short-term equine arteritis virus carrier (n=6) and long-term equine arteritis virus carrier stallions (n=3). Findings were further confirmed by RT-qPCR and other experiments were performed to characterize the inflammatory response and the involvement of the chemokine CXCL16 and its receptor CXCR6. Gene expression was also analyzed based on the CXCL16 genotype (CXCL16S or CXCL16R).
 
Contributor(s) Carossino M, Dini P, Kalbfleisch TS, Loynachan AT, Canisso IF, Cook RF, Bailey E, Timoney PJ, Balasuriya UB
Citation(s) 31356622
Submission date May 29, 2018
Last update date Sep 11, 2019
Contact name Udeni B. R. Balasuriya
E-mail(s) ubalasuriya@uky.edu
Phone 8592181124
Organization name University of Kentucky
Department Veterinary Science
Street address 108 Maxwell H. Gluck Equine Research Center
City Lexington
State/province KY
ZIP/Postal code 40546
Country USA
 
Platforms (1)
GPL21401 Illumina NextSeq 500 (Equus caballus)
Samples (12)
GSM3161940 L140
GSM3161941 L138
GSM3161942 L141
Relations
BioProject PRJNA473438
SRA SRP149123

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Supplementary file Size Download File type/resource
GSE114982_RAW.tar 8.7 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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