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Series GSE114931 Query DataSets for GSE114931
Status Public on Nov 21, 2018
Title Antigen-specific T-cell receptor signatures of cytomegalovirus infection
Organism Homo sapiens
Experiment type Other
Summary We immunosequenced peptide-stimulated PBMCs from 8 CMV-positive donors to identify virus-specific T cell receptors. We also sequenced ex vivo T cell repertoires of multiple CMV-postive and CMV-negative donors to compare the availability of CMV-specific TCRs in virus carriers and uninfected subjects.
Overall design Blood samples from healthy anonymous donors were obtained from the "Institut für Transfusionsmedizin Ulm ", Germany. PBMCs were isolated by standard Ficoll gradient centrifugation and mRNA was isolated either directly or after 10-day stimulation with an immunogenic CMV peptide.
For validation of our short-term stimulated TCRs, PBMCs from 3 different donors were stimulated with autologous transformed B cells (mini-LCLs) for 23 or 30 days. These mini-LCLs expressed one of the CMV antigens pp65 or IE1 together with EBV antigens, or EBV antigens alone (control mini-LCLs).
TCR-enriched sequencing libraries were obtained using TCR beta chain-specific primers with Illumina adapter sequence overhangs. After Sequencing on an Illumina HiSeq1500 device, raw data was demultiplexed, quality filtered, and TCR repertoires were generated by alignment and grouping of individual reads.
Contributor(s) Moosmann A, Huth A, Liang X, Krebs S, Blum H
Citation(s) 30587531
Submission date May 25, 2018
Last update date Feb 20, 2019
Contact name Andreas Moosmann
Phone +49 89 3187 1202
Organization name Helmholtz Center Munich
Department AGV
Street address Marchioninistr. 25
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (75)
GSM3155090 P01_unstim_CD8_beta
GSM3155091 P01_unstim_all_beta
GSM3155092 P01_CRVstim_CD8_beta
BioProject PRJNA473147
SRA SRP148992

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Supplementary file Size Download File type/resource
GSE114931_RAW.tar 294.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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