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Series GSE114918 Query DataSets for GSE114918
Status Public on Jun 21, 2021
Title RNAseq of human SNc and VTA midbrain dopamine neurons isolated from post mortem material of control subjects and Parkinson's Disease patients using laser capture microdisection.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Defining the transcriptional profiles of SNc and VTA dopamine neurons could reveal mechanisms underlying their differential susceptibilities in Parkinson’s disease. To conclusively identify genes stably enriched in either VTA or SNc dopamine neurons we conducted transcriptomic profiling across 18 human subjects and revealed novel genes in human that stably define dopamine neuron subtypes by random pooling algorithm.
Overall design A total of 50 samples were analyzed. Each sample consists of approximately 50-120 laser-captured motor neurons from post-mortem human tissue. Samples are divided in 4 groups (Control SNc, n=26; Control VTA, n=17; PD SNc, n=5; PD VTA, n=4). For cases having more than 1 replicate per group, corresponding samples were averaged before analysis so that each Case have only 1 SNc and 1 VTA.
Contributor(s) Benitez JA, Cheng S, Wang M, Deng Q, Hedlund E
Citation(s) 34305528
Submission date May 25, 2018
Last update date Jul 28, 2021
Contact name Eva Hedlund
Phone 0046761130911
Organization name Stockholm University
Department Department of Biochemistry and Biophysics
Lab Hedlund
Street address Svante Arrhenius v. 16C
City Stockholm
ZIP/Postal code 106 91
Country Sweden
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (52)
GSM3154492 case10_Substantia_Nigra_compacta_rep1
GSM3154493 case10_Substantia_Nigra_compacta_rep2
GSM3154494 case11_Substantia_Nigra_compacta
BioProject PRJNA473129
SRA SRP148970

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Supplementary file Size Download File type/resource
GSE114918_GEO_COUNTS_LCMseq_humanPK_SNc_VTA.txt.gz 244.5 Kb (ftp)(http) TXT
GSE114918_GEO_RPKMS_LCMseq_human_SNc_VTA.txt.gz 3.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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