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Series GSE114856 Query DataSets for GSE114856
Status Public on May 22, 2019
Title RNA sequencing results of EMT and CSC phenotypes induced by modulated expression of wild-type and mutated PSPC1 and PTK6
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We found that PSPC1 is the master activator for transcription factors of EMT and stemness and accompanies c-Myc activation to facilitate tumour growth. To elucidate mechanisms that the underlying post-translational modification of PSPC1, we performed the RNA-seq analysis of overexpression of PSPC1, alone and with PTK6, and PSPC1 tyrosine residue mutation in SK-Hep1 cells. We performed c-terminal of PSPC1 and its mutated nuclear localization site (NLS) in Mahlavu cells to evaluate its inhibitory effects on gene expression. Moreover, we further treated with HGF in Huh-7 cells to examineThe role of downstream signaling pathways of the PSPC1 and PTK6 regulation in microenviroment.
Overall design Gene expression profiles of PSPC1 with modulated overexpression in SK-Hep1 cells and Mahlavu cells were generated by Illumna RNA sequencing and compared to profiles derived from mock control cells. Gene expression profiles of HGF treatment in Huh-7 cells were generated by Illumna RNA sequencing and compared to profiles derived from mock control cells.
Web link
Contributor(s) Lang YD, Jou YS
Citation(s) 31844057
Submission date May 24, 2018
Last update date Dec 18, 2019
Contact name Yaw-dong lang
Organization name Academia Sinica
Department Institute of biomedical science
Street address 28 Sec. 2, Academia Rd. Nankang, Taipei 115
City Taipei, Taiwan
State/province Taiwan
ZIP/Postal code 115
Country Taiwan
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (10)
GSM3151910 M
GSM3151911 P
GSM3151912 A_P
BioProject PRJNA472874
SRA SRP148855

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114856_RAW.tar 9.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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