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| Status |
Public on May 22, 2019 |
| Title |
RNA sequencing results of EMT and CSC phenotypes induced by modulated expression of wild-type and mutated PSPC1 and PTK6 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We found that PSPC1 is the master activator for transcription factors of EMT and stemness and accompanies c-Myc activation to facilitate tumour growth. To elucidate mechanisms that the underlying post-translational modification of PSPC1, we performed the RNA-seq analysis of overexpression of PSPC1, alone and with PTK6, and PSPC1 tyrosine residue mutation in SK-Hep1 cells. We performed c-terminal of PSPC1 and its mutated nuclear localization site (NLS) in Mahlavu cells to evaluate its inhibitory effects on gene expression. Moreover, we further treated with HGF in Huh-7 cells to examineThe role of downstream signaling pathways of the PSPC1 and PTK6 regulation in microenviroment.
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| Overall design |
Gene expression profiles of PSPC1 with modulated overexpression in SK-Hep1 cells and Mahlavu cells were generated by Illumna RNA sequencing and compared to profiles derived from mock control cells. Gene expression profiles of HGF treatment in Huh-7 cells were generated by Illumna RNA sequencing and compared to profiles derived from mock control cells.
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| Web link |
https://www.nature.com/articles/s41467-019-13665-6
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| |
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| Contributor(s) |
Lang YD, Jou YS |
| Citation(s) |
31844057 |
| |
| Submission date |
May 24, 2018 |
| Last update date |
Dec 18, 2019 |
| Contact name |
Yaw-dong lang |
| E-mail(s) |
lang9161@gmail.com
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| Organization name |
Academia Sinica
|
| Department |
Institute of biomedical science
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| Street address |
28 Sec. 2, Academia Rd. Nankang, Taipei 115
|
| City |
Taipei, Taiwan |
| State/province |
Taiwan |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA472874 |
| SRA |
SRP148855 |
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