GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE114436 Query DataSets for GSE114436
Status Public on May 14, 2019
Title RNA sequencing in hepatocytes and DR-derived hepatocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During chronic liver injury, the ductular reaction generates hepatocytes. The aim of the analysis is to compare the phenotype of the DR-derived hepatocytes with that of native hepaotcytes in the same injured liver and control healthy hepatocytes.
Overall design We compared 5 biological replicates of each of the 3 populations. Due to technical constraints 10 samples were processed in a first experiment (Experiment 1) and 9 samples were processed in a second experiment (experiment 2). Log fold-change obtained from both experiment were highly correlated (r ~ 0.95).

Several of the same samples were processed in both Experiment 1 and Experiment 2: CTL 5, RED 2, RED 3, RED 4. For these samples, independent libraries were made for each Experiment following methods outlined in the Extraction protocol on the sample pages.
Contributor(s) Ambroise J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 14, 2018
Last update date May 14, 2019
Contact name Jérôme Ambroise
Organization name Université catholique de Louvain
Department Institut de Recherche Expérimentale et Clinique
Street address Avenue Hippocrate 54
City Woluwé
ZIP/Postal code 1200
Country Belgium
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (19)
GSM3141686 Experiment 1 native Healthy hepatocytes - CTL 3
GSM3141687 Experiment 1 native Healthy hepatocytes - CTL 4
GSM3141688 Experiment 1 native Healthy hepatocytes - CTL 5
BioProject PRJNA471341
SRA SRP145671

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114436_Experiment1.txt.gz 818.2 Kb (ftp)(http) TXT
GSE114436_Experiment2.txt.gz 861.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap