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Series GSE114425 Query DataSets for GSE114425
Status Public on Sep 21, 2018
Title CD95L mRNA is toxic to cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary >80% of a large number of tested siRNAs and shRNAs targeting CD95 or CD95 ligand (CD95L) induce a form of cell death that is characterized by the simultaneous activation of multiple death pathways and preferentially affects transformed and cancer stem cells. We now show that these si/shRNAs kill cancer cells through canonical RNAi by targeting the 3’UTR of critical survival genes in a unique form of off-target effect. We also provide evidence showing that the full length CD95L mRNA is also toxic and produces small Ago-associated RNAs.
Overall design Pan Argonaute immunoprecipitation and small RNA cloning in HeyA8 and HCT116 wild-type and HCT116 Drosha KO cells treated with plenti or hFasL
Contributor(s) Putzbach WE, Haluck-Kangas A, Gao QQ, Sarshad AA, Bartom E, Stults A, Qadir AS, Scholtens DM, Hafner M, Peter ME
Citation(s) 30324908
Submission date May 14, 2018
Last update date Dec 23, 2018
Contact name Markus Hafner
Phone 3014026956
Organization name NIH
Department NIAMS
Lab Markus Hafner
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (12)
GSM3141533 miRNA_Drosha_hFasL1
GSM3141534 miRNA_Drosha_hFasL2
GSM3141535 miRNA_Drosha_plenti1
BioProject PRJNA471325
SRA SRP145632

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Supplementary file Size Download File type/resource
GSE114425_miRNAseq_HeyA8_HCT116_plenti_hFasL.xlsx 5.6 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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