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Series GSE11393 Query DataSets for GSE11393
Status Public on Jun 03, 2008
Title Monocyte gene expression profiling in familial combined hyperlipidemia and its modification by atorvastatin treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Introduction: The genetic origin of familial combined hyperlipidemia (FCH) is not well understood. We used microarray profiling of peripheral blood monocytes to search novel genes and pathways involved in FCH. Methods: Fasting plasma for determination of lipid profiles, inflammatory molecules, and adipokines was obtained and peripheral blood monocytes were isolated from male FCH patients basally and after 4 weeks of atorvastatin treatment. Sex-, age- and adiposity-matched controls were also studied. Gene expression profile was analyzed using Affymetrix Human Genome U133A 2.0 GeneChip arrays. Results: Analysis of gene expression by cDNA microarrays showed that 82 genes were differentially expressed in FCH monocytes compared to controls. Atorvastatin treatment modified the expression of 87 genes. Changes in the expression of some genes, confirmed by real time RT-PCR, (CD36, leucine-rich repeats and immunoglobulin-like domains-1, tissue factor pathway inhibitor 2, myeloid cell nuclear differentiation antigen tumor necrosis factor receptor superfamily, member 25 and CD96) may be related to a proinflammatory environment in FCH monocytes, which is partially reversed by atorvastatin. Higher plasma levels of triglycerides and free fatty acids and lower levels of adiponectin in FCH patients could also trigger changes in gene expression that atorvastatin cannot modify. Conclusions: Our results demonstrate clear differences in gene expression in FCH monocytes compared with those of matched healthy controls, some of which are influenced by atorvastatin treatment.
Keywords: comparative study differential gene expression
 
Overall design Peripheral blood monocytes were isolated from male FCH patients basally and after 4 weeks of atorvastatin treatment. Sex-, age- and adiposity-matched controls were also studied. Gene expression profile was analyzed using Affymetrix Human Genome U133A 2.0 GeneChip arrays (3 replicates for each condition)
 
Contributor(s) Llaverias G, Pou J, Ros E, Zambón D, Cofán M, Sánchez A, Vázquez-Carrera M, Sánchez RM, Laguna JC, Alegret M
Citation(s) 18681780
Submission date May 09, 2008
Last update date Dec 06, 2018
Contact name Marta Alegret
E-mail(s) alegret@ub.edu
Organization name University of Barcelona
Street address Diagonal 643
City Barcelona
ZIP/Postal code 08028
Country Spain
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (9)
GSM287664 Monocyte control rep 1
GSM287665 Monocyte control rep 2
GSM287666 Monocyte control rep 3
Relations
BioProject PRJNA106517

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11393_RAW.tar 17.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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