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Series GSE113858 Query DataSets for GSE113858
Status Public on Sep 08, 2020
Title Next Generation Sequencing Facilities Quantitative Analysis of Wild type CD8+ T cells with 0.3T static magnetic field or not
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose:Next Generation Sequencing applying for compare the CD8+ T cells with 0.3T static magnetic field (SMF) or not transcriptome, included pathway-enrichment analysis and genes expression of interests
Methods:CD8+ T cells were isolated from splenocytes in 8 week old wild type mice through STEMCELL Technologies negative selection kit. FACS sort CD8+ T cells after 3 days with CD3/CD28 costimulation at 0.3T static magnetic field or not. RNA was extracted, purified and checked for integrity using an Agilent Bioanalyzer 2100. Libraries were generated for sequencing using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. Libraries were sequenced using an Illumina HiSeq X Ten sequencer.
Resluts: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to further analyse
Conclusions:Our study represents the detailed analysis of CD8+ T cells with 0.3T static magnetic field or not transcriptomes
 
Overall design CD8+ T cells with 0.3T static magnetic field or not mRNA profiles of 8 week old wild type mice were generated by deep sequencing

Samples were replaced on October 5th, 2020.
 
Contributor(s) Zhu X, Liu Y
Citation(s) 32884074
Submission date Apr 30, 2018
Last update date Oct 06, 2020
Contact name liu yan
E-mail(s) liuyan1@shanghaitech.edu.cn
Phone 15821996287
Organization name shanghai institute of biochemistry and cell biology
Street address yueyang road 320
City shanghai
ZIP/Postal code 200000
Country China
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM4817605 Unstimulated sample1
GSM4817606 Unstimulated sample2
GSM4817607 SMF sample1
Relations
BioProject PRJNA454272
SRA SRP144021

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113858_RAW.tar 68.7 Mb (http)(custom) TAR (of GTF)
GSE113858_integrate_gene_expression_FPKM.txt.gz 1.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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