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Series GSE113805 Query DataSets for GSE113805
Status Public on Apr 28, 2018
Title Deregulated MicroRNAs in Cancer-Associated Fibroblasts from Front Tumor Tissues of Lung Adenocarcinoma as Potential Predictors of Tumor Promotion
Organism synthetic construct
Experiment type Non-coding RNA profiling by array
Summary Cancer-associated fibroblasts (CAFs) promote tumor progression through several mechanisms. MicroRNAs (miRNAs) play a key role in CAFs tumor-promoting properties, however, their role in CAFs from different topological areas in lung cancer progression remains unclear. This study aimed to characterize the miRNA expression profile of fibroblasts isolated from matched tumor front (F-CAFs), inner tumor (In-CAFs), and normal adjacent tissue (NFs) in lung adenocarcinoma, using microarray analysis and RT-qPCR. Proliferation and invasion assays of A549 lung cancer cells were performed in the presence of conditioned medium from F-CAFs, In-CAFs and NFs. Pathway enrichment analysis and miRNA-target networks were performed to identify tumorigenesis-related miRNAs. Both F-CAFs and In-CAFs enhanced the proliferation and invasion of A549 cells compared to NFs; however, F-CAFs showed a significantly stronger effect than In-CAFs. qPCR demonstrated three downregulated miRNAs in F-CAFs versus NFs (miR-145-3p, miR-299-3 p, miR-505-3p) and two in F-CAFs versus In-CAFs (miR-410-3p, miR-485-5p). Deregulated miRNAs showed significant association to “pathways in cancer”, “Wnt signaling pathway”, and “TGF-beta signaling pathway”, targeting tumor-promoting growth factors as IGF1 and VEGFA. Our findings suggest that deregulated miRNAs in F-CAFs, which showed the strongest effect on the invasive and proliferative capacity of A546 cancer cells, present potential predictive association with tumor-promoting properties.
Overall design Tissue samples from the tumor front (F-CAF, 4 samples), matched inner tumor tissue (In-CAF, 4 samples), and matched adjacent normal (NF, 4 samples) lung tissue from each patient with primary NSCLC subtype adenocarcinoma (tumor stage 2) were obtained by surgical resection at the Instituto Nacional de Enfermedades Respiratorias; RNA extraction and hybridization on miRNA 4.0 Affymetrix microarray were performed.
Contributor(s) Negrete-Garcia MC, Ramírez-Rodriguez SL, Rangel-Escareño C, Muñoz-Montero SA, Kelly-García J, Vázquez-Manríquez ME, Santillán P, Montaño Ramírez M, Ramírez-Martínez G, Ramírez-Venegas A, Ortiz-Quintero B
Citation(s) 30369556
Submission date Apr 27, 2018
Last update date Oct 31, 2018
Contact name Claudia Rangel Escareño
Organization name INMEGEN
Department Computational Genomics
Street address Periferico Sur 4809
City Tlalpan
State/province Mexico City
ZIP/Postal code 01090
Country Mexico
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (12)
GSM3119651 Tumor front CAF, biological replicate 1
GSM3119652 Tumor front CAF, biological replicate 2
GSM3119653 Tumor front CAF, biological replicate 3
BioProject PRJNA454011

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Supplementary file Size Download File type/resource
GSE113805_RAW.tar 9.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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