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Series GSE113537 Query DataSets for GSE113537
Status Public on Apr 23, 2021
Title Genome-wide DNA methylation maps of Decitabine and/or Cisplatin treated bladder cancer cells.
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary It is well established that low-dose Decitabine treatment can sustain long-term antitumor effects by targeting DNA methylation and altering gene expression32. We performed methylated DNA immunoprecipitation (MEDIP) followed by next-generation sequencing in 5637 cells. Daily Decitabine treatment with 100 nM for 72 hr (with or without 100ng/ml cisplatin treatment) reduced the methylation level at CpG-islands in the genome of 5637 cells . However, this low-dose DEC treatment did not cause a significant reduction of DNA methylation in non-CpG islands regions.Our study represents the first detailed analysis of DNA methylome generated by RNA-seq technology in bladder cancer cells treated with low-dose decitabine. This study help to reveal the direct target genes of decitabine in bladder cancer treatement.
Overall design Examination of DNA methylation level of bladder cancer cells treated with lose-dose decitabine.
Contributor(s) Li Y, Li J
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Submission date Apr 23, 2018
Last update date Apr 24, 2021
Contact name Jiong Li
Phone 3105617186
Organization name UCLA
Department School of Dentistry
Lab Cunyu Wang
Street address 33-030 CHS
City Los Angeles
State/province CA - California
ZIP/Postal code 91406
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM3108730 Input Mock
GSM3108731 Mock
GSM3108732 Input Decitabine
BioProject PRJNA451473
SRA SRP142314

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113537_RAW.tar 502.4 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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