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Series GSE113326 Query DataSets for GSE113326
Status Public on Jul 02, 2018
Title Epigenetic reprogramming in hepatic carcinogenesis
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Genomic sequencing of hepatocellular carcinoma (HCC) uncovers a paucity of actionable mutations, underscoring the necessity to exploit epigenetic vulnerabilities for therapeutics. In HCC, EZH2-mediated H3K27me3 represents a major oncogenic chromatin modification, but how it modulates the therapeutic vulnerability of signaling pathways remains unknown. Here, we identified that EZH2 maintains H3K27 methylome through epigenetic silencing of specific gene sets. ChIP-seq revealed enrichment of EZH2/H3K27me3 at silenced loci in HBx-transgenic (TG) mouse-derived HCCs, including tumor suppressors whose down-regulation significantly correlated with EZH2 overexpression and poor survival of HCC patients. Defining the aberrant chromatin landscape of HCC sheds light into the mechanistic basis of effective EZH2-targeted inhibition.
Overall design The normal liver (NL) tissue from 3 month-old wild-type (WT) C57BL/6 mouse, and the non-tumor (NT) and tumor (T) tissues from 18 month-old HBx-TG mice were collected for ChIP-seq analyses using antibodies targeting EZH2 and H3K27me3. The genome-wide enrichment of EZH2 and H3K27me3 in T tissue was compared against those in NL and NT tissues, respectively, as to identify the epigenentically silenced gene sets unique to HCCs.
Contributor(s) Lee Y, Mok MT, Wu F, Cheng AS
Citation(s) 29992318
Submission date Apr 18, 2018
Last update date Oct 03, 2018
Contact name Alfred Sze-Lok Cheng
Organization name The Chinese University of Hong Kong
Department School of Biomedical Sciences
Street address Rm 405A, Lo Kwee-Seong Integrated Biomedical Sciences Building, Area 39, CUHK
City Hong Kong
ZIP/Postal code 0000
Country Hong Kong
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (10)
GSM3103275 TG-18m-NT-EZH2
GSM3103276 TG-18m-NT-Input1
GSM3103277 TG-18m-T-EZH2
BioProject PRJNA450821
SRA SRP140915

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Supplementary file Size Download File type/resource
GSE113326_RAW.tar 3.8 Gb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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