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Series GSE113257 Query DataSets for GSE113257
Status Public on May 09, 2018
Title Integrative analysis of gut microbiota composition, host colonic gene expression and intraluminal metabolites in aging C57BL/6J mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Diminished colonic health is associated with various age-related pathologies. In this study, we applied an integrative approach to reveal potential interactions between determinants of colonic health in aging C57BL/6J mice. Analysis of gut microbiota composition revealed an enrichment of various potential pathobionts, including Desulfovibrio spp., and a decline of the health-promoting Akkermansia spp. and Lactobacillus spp. during aging. Intraluminal concentrations of various metabolites varied between ages and we found evidence for an increased gut permeability at higher age. Colonic gene expression analysis suggested that during the early phase of aging (between 6 and 12 months), expression of genes involved in epithelial-to-mesenchymal transition and (re)organization of the extracellular matrix were increased. Differential expression of these genes was strongly correlated with Bifidobacterium spp. During the later phase of aging (between 12 and 28 months), gene expression profiles pointed towards a diminished antimicrobial defense and were correlated with an uncultured Gastranaerophilales spp. This study demonstrates that aging is associated with pronounced changes in gut microbiota composition and colonic gene expression. Furthermore, the strong correlations between specific bacterial genera and host gene expression may imply that orchestrated interactions take place in the vicinity of the colonic wall and potentially mediate colonic health during aging.
Overall design Male C57BL/6J mice were randomly distributed into four groups that were sacrificed at 6, 12, 24 and 28 months. Mice were housed individually and fed an ad libitum semi-synthetic (AIN-93W) control diet. Upon sacrifice at 6, 12, 24 or 28 months of age, colonic scrapings were collected and RNA was isolated for microarray analyses.
Contributor(s) van der Lugt B, Rusli F, Lute C, Lamprakis A, Salazar E, Boekschoten M, Hooiveld GJ, Müller M, Vervoort J, Kersten S, Belzer C, Kok D, Steegenga WT
Citation(s) 29769431
Submission date Apr 17, 2018
Last update date Aug 13, 2018
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (26)
GSM3101204 colon, control diet, age 24 months, rep15
GSM3101205 colon, control diet, age 28 months, rep19*
GSM3101206 colon, control diet, age 28 months, rep21*
BioProject PRJNA450595

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Supplementary file Size Download File type/resource
GSE113257_RAW.tar 123.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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