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Series GSE112833 Query DataSets for GSE112833
Status Public on May 01, 2019
Title SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development [Bisulfite-seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary The oocyte epigenome plays critical roles in mammalian gametogenesis and embryogenesis. Yet, how it is established remains elusive. Here, we report that histone-lysine N-methyltransferase SETD2, an H3K36me3 methyltransferase, is a crucial regulator of the mouse oocyte epigenome. Deficiency in Setd2 leads to extensive alterations of the oocyte epigenome, including the loss of H3K36me3, failure in establishing the correct DNA methylome, invasion of H3K4me3 and H3K27me3 into former H3K36me3 territories and aberrant acquisition of H3K4me3 at imprinting control regions instead of DNA methylation. Importantly, maternal depletion of SETD2 results in oocyte maturation defects and subsequent one-cell arrest after fertilization. The preimplantation arrest is mainly due to a maternal cytosolic defect, since it can be largely rescued by normal oocyte cytosol. However, chromatin defects, including aberrant imprinting, persist in these embryos, leading to embryonic lethality after implantation. Thus, these data identify SETD2 as a crucial player in establishing the maternal epigenome that in turn controls embryonic development.
Overall design Mouse GO_P7, GO_P10, GO_P14, FGO, MII oocyte, zygote, late 2-cell, 8-cell and ICM from E3.5 blastocyst were obtained and performed STAR ChIP-seq to determine the H3K36me3 distribution. FGO in Dnmt3L KO mice and Setd2 maternal KO mice were collected and conducted STAR ChIP-seq, STEM-seq and RNA-seq to explore the cross talk between histone modifications. STAR ChIP-seq and RNA-seq were performed in Setd2 maternal KO zygote to test if abnormal H3K4me3 and transcription can be inherited in mutant embryo.
GO = growing oocyte
FGO = full grown oocyte
Contributor(s) Xu Q, Xiang Y, Xie W
Citation(s) 31040401
Submission date Apr 06, 2018
Last update date May 17, 2019
Contact name Wei Xie
Organization name Tsinghua University
Department School of Life Science
Street address Haidian District
City Beijing
ZIP/Postal code 100084
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (11)
GSM3084608 MethylC_FGO_Dnmt3l_Con_5mC_rep1
GSM3084609 MethylC_FGO_Dnmt3l_Con_5mC_rep2
GSM3084610 MethylC_FGO_Dnmt3l_KO_5mC_rep1
This SubSeries is part of SuperSeries:
GSE112835 SETD2 regulates the maternal epigenome, genomic imprinting and embryonic development
BioProject PRJNA449121
SRA SRP137906

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Supplementary file Size Download File type/resource
GSE112833_5mC.bed.tar.gz 48.5 Mb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record

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