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Series GSE112737 Query DataSets for GSE112737
Status Public on Apr 06, 2018
Title Piwi knockdown in Drosophila Intestinal Stem Cells
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Transcriptome analysis of Piwi-deficient ISCs isolated by FACS. We used esg::Gal4, UAS::GFP; tub::Gal80ts to drive the expression of an RNAi against mCherry (control) or Piwi for 7 days.
Overall design Duplicates of mCherry RNAi expressing ISCs and Piwi RNAi expressing ISCs. Each sample was obtained from a pool of 100 dissected and FACSorted fly guts.
Contributor(s) Sousa-Victor P, Jasper H
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Submission date Apr 05, 2018
Last update date Feb 20, 2019
Contact name Pedro Sousa-Victor
Phone 4152023045
Organization name Buck Institute for Research on Aging
Lab Jasper Lab
Street address 8001 Redwood Blvd.
City Novato
State/province CALIFORNIA
ZIP/Postal code 94945
Country USA
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (4)
GSM3082756 WT1: mCherry RNAi 7 days 1
GSM3082757 WT2: mCherry RNAi 7 days 2
GSM3082758 PIWI1: Piwi RNAi 7 days 1
BioProject PRJNA448884
SRA SRP137690

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112737_RNAseq_Sousaetal_submit_Piwi.xlsx 1.2 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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