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| Status |
Public on Jun 04, 2020 |
| Title |
A genome-wide screen identifies E2F6 as critical factor for TMZ chemoresistance in EGFRvIII expressing GBM (CRISPR library RNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We conducted a genome-wide screen by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 library to identify genes which are particularly resistant to EGFRvIII GBM cells under the chemo-stress of TMZ. In vitro and in vivo validation of EGFRvIII-specific targets revealed several strong hits, including E2F6. Mechanistic studies demonstrated that E2F6 reveals its resistance to TMZ through NF-κB and TMZ inducement.EGFRvIII GBM cells under the chemo-stress of TMZ.
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| |
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| Overall design |
Examination of TMZ resistant and sensitive genes in U87EGFRvIII cells, each with 3 replicates.
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| |
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| Contributor(s) |
Kang C |
| Citation(s) |
31508283 |
| |
| Submission date |
Apr 05, 2018 |
| Last update date |
Jun 04, 2020 |
| Contact name |
Kai Huang |
| E-mail(s) |
huangkai@tijmu.edu.cn
|
| Phone |
13752390609
|
| Organization name |
tianjin medical university
|
| Street address |
anshan roud
|
| City |
tianjin |
| ZIP/Postal code |
300054 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE112736 |
A genome-wide screen identifies E2F6 as critical factor for TMZ chemoresistance in EGFRvIII expressing GBM |
|
| Relations |
| BioProject |
PRJNA448879 |
| SRA |
SRP137674 |
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