GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE112590 Query DataSets for GSE112590
Status Public on Dec 19, 2019
Title Silencing of BEX2 promotes colorectal cancer metastasis through Hedgehog signaling pathway
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Colorectal cancer is one of the most common cancers worldwide with increasing incidence, the presence of metastasis is one of the major causes for poor outcome. BEX2 has been reported to be involved in tumor development in several types of cancer, but is poorly understood in metastatic colorectal cancer. Here we demonstrated that knockout of BEX2 resulted in the enhancement of the migratory and metastatic potential of colorectal cancer cells in vivo and in vitro, re-expression of BEX2 in knockout cells could reverse the migratory enhancement. Expression profile chip indicated that hedgehog signaling pathway was activated after knockout of BEX2, and hedgehog Signaling inhibitor GANT61 and GDC-0449 could somehow reverse the migratory enhancement of BEX2-/- colorectal cancer cells. We also demonstrated that it is the nucleus translocation of Zic2 after BEX2 silenced, that activated hedgehog signaling pathway, while knockdown Zic2 could also abrogated migratory enhancement of BEX2-/- cells. In summary, our findings suggest that BEX2 is a negative modulator of hedgehog signaling pathway by retaining Zic2 in the cytoplasm of colorectal cancer cells, thus to inhibit colorectal cancer cell migration and metastasis.
Overall design mRNA profiles of colorectal cancer cell DLD1 (WT) and BEX2 knocked out DLD1 cells (DLD1 KO1 and DLD1 KO2) were generated by deep sequencing, using Illumina Hiseq 3000.
Contributor(s) Tan Y, Hu Y, Xiao Q, Tang Y, Chen H, He J, Chen L, Jiang K, Wang Z, Liu Y, Chen L, Sun L, Yuan Y, Ding K
Citation(s) 31929751
Submission date Apr 02, 2018
Last update date Mar 28, 2020
Contact name Kefeng Ding
Organization name The Second Affiliated Hospital of Zhejiang University School of Medicine
Department Department of Colorectal Surgery
Street address 88 Jiefang Road
City Hangzhou
State/province Zhejiang Province
ZIP/Postal code 310000
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (3)
GSM3073358 DLD1
GSM3073359 DLD1 KO1
GSM3073360 DLD1 KO2
BioProject PRJNA448418
SRA SRP136930

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112590_gene_sample_FPKM.txt.gz 670.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap