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Series GSE112508 Query DataSets for GSE112508
Status Public on Aug 31, 2020
Title Optical stimulation of the FGF signaling pathway is sufficient to maintain human pluripotent stem cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Stem cell fate is largely determined by a cell-signaling network and can be controlled by the supplementation of exogenous recombinant proteins; this, however, may cause heterogeneous and unsynchronized signaling due to the uneven distribution of recombinant proteins. Such issues are closely associated with the spontaneous differentiation of human pluripotent stem cells (hPSC), which lead to a continuing loss of pluripotency. We report a novel optical control system to maintain the cellular fate of hPSCs without the daily supplementation of recombinant Fibroblast Growth Factor 2 (FGF2) protein, a key molecule for their stemness. Using blue light illumination, we mimick the activation of the FGF signaling pathway in hPSCs carrying the large light-oxygen-voltage (LOV)-sensing domain, an algae-/plant-derived photo-activable protein. The optically maintained hPSCs have similar cellular and molecular profiles to those cultured with FGF2 protein and display differentiation capabilities into three germ layers. These data provide proof-of-concept that the optical control of signaling pathways can be applied to human stem cells.
Overall design We established an optical control system to maintain the characterization of hPSCs with blue light illumination. It was possible to maintain the status of undifferentiation with typical stemness abilities. Furthermore, we used RNA-seq for the comparison of protein treatment and blue light illumination groups to observe gene expression profiles in triplicate.
Contributor(s) Gabsang L, Hyesoo K, In Young C
Citation(s) 32736809
Submission date Mar 29, 2018
Last update date Nov 30, 2020
Contact name Gabsang Lee
Organization name Institute for Cell Engineering, Johns Hopkins University
Department Department of Neurology and Neuroscience
Street address 733 North Broadway, Suite 747
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (15)
GSM3071618 Opto-10min-1
GSM3071619 Opto-10min-2
GSM3071620 Opto-10min-3
BioProject PRJNA448012
SRA SRP136740

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112508_RAW.tar 2.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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