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| Status |
Public on Aug 31, 2020 |
| Title |
Optical stimulation of the FGF signaling pathway is sufficient to maintain human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stem cell fate is largely determined by a cell-signaling network and can be controlled by the supplementation of exogenous recombinant proteins; this, however, may cause heterogeneous and unsynchronized signaling due to the uneven distribution of recombinant proteins. Such issues are closely associated with the spontaneous differentiation of human pluripotent stem cells (hPSC), which lead to a continuing loss of pluripotency. We report a novel optical control system to maintain the cellular fate of hPSCs without the daily supplementation of recombinant Fibroblast Growth Factor 2 (FGF2) protein, a key molecule for their stemness. Using blue light illumination, we mimick the activation of the FGF signaling pathway in hPSCs carrying the large light-oxygen-voltage (LOV)-sensing domain, an algae-/plant-derived photo-activable protein. The optically maintained hPSCs have similar cellular and molecular profiles to those cultured with FGF2 protein and display differentiation capabilities into three germ layers. These data provide proof-of-concept that the optical control of signaling pathways can be applied to human stem cells.
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| Overall design |
We established an optical control system to maintain the characterization of hPSCs with blue light illumination. It was possible to maintain the status of undifferentiation with typical stemness abilities. Furthermore, we used RNA-seq for the comparison of protein treatment and blue light illumination groups to observe gene expression profiles in triplicate.
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| Contributor(s) |
Gabsang L, Hyesoo K, In Young C |
| Citation(s) |
32736809 |
| Submission date |
Mar 29, 2018 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Gabsang Lee |
| E-mail(s) |
glee48@exchange.johnshopkins.edu
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| Organization name |
Institute for Cell Engineering, Johns Hopkins University
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| Department |
Department of Neurology and Neuroscience
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| Street address |
733 North Broadway, Suite 747
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA448012 |
| SRA |
SRP136740 |
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