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| Status |
Public on Apr 01, 2010 |
| Title |
Peripheral blood stem cell microRNA profiling |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; human gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus macacae |
| Sample organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Mobilized-peripheral blood hematopoietic stem cells (HSCs) have been used for transplantation, immunotherapy, and cardiovascular regenerative medicine. Agents used for HPC mobilization include G-CSF and the CXCR4 inhibitor AMD3100. The HSCs cells mobilized by each agent may contain different subtypes and have different functions. To characterize mobilized HSCs used for clinical applications, microRNA (miRNA) profiling and gene expression profiling were used to compare AMD3100-mobilized CD133+ cells from 4 subjects, AMD3100 plus G-CSF-mobilized CD133+ cells from 4 subjects and G-CSF-mobilized CD34+ cells from 5 subjects. The HSCs were compared to peripheral blood leukocytes (PBLs) from 7 subjects.
Keywords: cell type comparison design
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|
| Overall design |
microRNA (miRNA) profiling were used to compare AMD3100-mobilized CD133+ cells from 4 subjects, AMD3100 plus G-CSF-mobilized CD133+ cells from 4 subjects and G-CSF-mobilized CD34+ cells from 5 subjects. The HSCs were compared to peripheral blood leukocytes (PBLs) from 7 subjects.
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| |
|
| Citation(s) |
18647411 |
| |
| Submission date |
Apr 23, 2008 |
| Last update date |
May 25, 2012 |
| Contact name |
Francesco Maria Marincola |
| E-mail(s) |
fmarincola@sidra.org
|
| Phone |
301-793-8210
|
| Organization name |
Sidra Medical and Research Center
|
| Street address |
Al Nasr Tower, AL Corniche Street, PO Box 26999
|
| City |
Doha |
| ZIP/Postal code |
PO Box 26999 |
| Country |
Qatar |
| |
|
| Platforms (1) |
|
| Samples (41)
|
| GSM283993 |
D1-CD133-G-CSF+AMD3100 - mAdbID:82732 |
| GSM283994 |
D2-CD133-G-CSF+AMD3100 - mAdbID:82733 |
| GSM283995 |
D3-CD133-AMD3100 - mAdbID:82734 |
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| This SubSeries is part of SuperSeries: |
| GSE11255 |
Peripheral blood stem cell gene and microRNA profiling |
|
| Relations |
| BioProject |
PRJNA109191 |
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