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| Status |
Public on Mar 28, 2019 |
| Title |
The long noncoding RNA lnc-ob1 facilitates bone formation by upregulating Osterix in osteoblasts |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Long noncoding RNAs (lncRNAs) have emerged as integral regulators of physiology and disease, but specific roles of lncRNAs in bone disease remain largely unknown. Here, we show that lnc-ob1 regulates osteoblast activity and bone formation in mice by upregulating the osteogenic transcription factor Osterix. Expression of lnc-ob1 is enriched in osteoblasts and upregulated during osteoblastogenesis. We demonstrate that osteoblast-specific knock-in of lnc-ob1 enhances bone formation and increases bone mass. Pharmacological overexpression of lnc-ob1 specifically in osteoblasts confers resistance to ovariectomy-induced osteoporosis in mice. In humans, expression of the homologue, lnc-OB1, decreases with age in osteoblasts of patients with osteoporosis. Mechanistically, lnc-ob1 upregulates the expression of Osterix in mouse and human osteoblasts, probably via inhibition of H3K27me3 methylation. Our data indicate that lnc-OB1 regulates bone formation and might be a drug target for the treatment of osteoporosis.
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| Overall design |
High throughput RNA-seq was performed on human and mouse undifferentiated BMSCs (0 week) and osteoblastic differentiated BMSCs (2 weeks). RNA-seq was also performed on the osteoblast isolated from lnc-OB1 knockdown KI mice and N.C control KI mice.
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| Contributor(s) |
Sun Y, Cai M, Zhong J, Yang L, Xiao J, Jin F, Xue H, Liu X, Liu H, Zhang Y, Jiang D, Hong A, Ji X, Wang Z, Zhang G, Wang X |
| Citation missing |
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| Submission date |
Mar 26, 2018 |
| Last update date |
Mar 29, 2019 |
| Contact name |
Gong Zhang |
| E-mail(s) |
zhanggong@jnu.edu.cn
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| Organization name |
Jinan University
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| Department |
Institute of Life and Health Engineering
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| Lab |
Translatomics Lab
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| Street address |
Huang-Pu Avenue West 601
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| City |
Guangzhou |
| ZIP/Postal code |
510632 |
| Country |
China |
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| Platforms (2) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA445648 |
| SRA |
SRP136440 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112318_RAW.tar |
9.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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