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Series GSE112318 Query DataSets for GSE112318
Status Public on Mar 28, 2019
Title The long noncoding RNA lnc-ob1 facilitates bone formation by upregulating Osterix in osteoblasts
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Long noncoding RNAs (lncRNAs) have emerged as integral regulators of physiology and disease, but specific roles of lncRNAs in bone disease remain largely unknown. Here, we show that lnc-ob1 regulates osteoblast activity and bone formation in mice by upregulating the osteogenic transcription factor Osterix. Expression of lnc-ob1 is enriched in osteoblasts and upregulated during osteoblastogenesis. We demonstrate that osteoblast-specific knock-in of lnc-ob1 enhances bone formation and increases bone mass. Pharmacological overexpression of lnc-ob1 specifically in osteoblasts confers resistance to ovariectomy-induced osteoporosis in mice. In humans, expression of the homologue, lnc-OB1, decreases with age in osteoblasts of patients with osteoporosis. Mechanistically, lnc-ob1 upregulates the expression of Osterix in mouse and human osteoblasts, probably via inhibition of H3K27me3 methylation. Our data indicate that lnc-OB1 regulates bone formation and might be a drug target for the treatment of osteoporosis.
Overall design High throughput RNA-seq was performed on human and mouse undifferentiated BMSCs (0 week) and osteoblastic differentiated BMSCs (2 weeks). RNA-seq was also performed on the osteoblast isolated from lnc-OB1 knockdown KI mice and N.C control KI mice.
Contributor(s) Sun Y, Cai M, Zhong J, Yang L, Xiao J, Jin F, Xue H, Liu X, Liu H, Zhang Y, Jiang D, Hong A, Ji X, Wang Z, Zhang G, Wang X
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Submission date Mar 26, 2018
Last update date Mar 29, 2019
Contact name Gong Zhang
Organization name Jinan University
Department Institute of Life and Health Engineering
Lab Translatomics Lab
Street address Huang-Pu Avenue West 601
City Guangzhou
ZIP/Postal code 510632
Country China
Platforms (2)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3067604 hBMSC_0_week
GSM3067605 hBMSC_2_week
GSM3067606 mBMSC_0_week
BioProject PRJNA445648
SRA SRP136440

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Supplementary file Size Download File type/resource
GSE112318_RAW.tar 9.6 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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