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Series GSE112289 Query DataSets for GSE112289
Status Public on Jun 04, 2018
Title Extracellular RNA profiles with human age
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Circulating extracellular RNAs (exRNAs) are potential biomarkers of disease. We thus hypothesized that age-related changes in exRNAs can identify age-related processes. We profiled both large and small RNAs in human serum to investigate changes associated with normal aging. exRNA was sequenced in 13 young (30-32 yrs.) and 10 old (80-85 yrs.) African American women to identify all RNA transcripts present in serum. We identified age-related differences in several RNA biotypes, including mitochondrial transfer RNAs, mitochondrial ribosomal RNA, and unprocessed pseudogenes. Age-related differences in unique RNA transcripts were further validated in an expanded cohort. Pathway analysis revealed that EIF2 signaling, oxidative phosphorylation, and mitochondrial dysfunction were among the top pathways shared between young and old. Protein interaction networks revealed distinct clusters of functionally-related protein-coding genes in both age-groups. These data provide timely and relevant insight into the exRNA repertoire in serum and its change with aging.
Overall design Profiling of extracellular RNA (exRNA) from human serum in 13 young (30.9 ± 0.60 yrs) and 10 old (81.8 ± 1.87 yrs) individuals.
Contributor(s) Dluzen DF, Noren Hooten N, De S, Wood III WH, Zhang Y, Becker KG, Zonderman AB, Tanaka T, Ferrucci L, Evans MK
Citation(s) 29797538
Submission date Mar 23, 2018
Last update date Jun 22, 2020
Contact name Supriyo De
Organization name NIA-IRP, NIH
Department Laboratory of Genetics and Genomics
Lab Computational Biology & Genomics Core
Street address 251 Bayview Blvd
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (23)
GSM3066231 G01S01Y: Young 1
GSM3066232 G01S02Y: Young 2
GSM3066233 G01S04O: Old 1
BioProject PRJNA445476
SRA SRP136360

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Supplementary file Size Download File type/resource
GSE112289_RAW.tar 3.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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