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Series GSE112175 Query DataSets for GSE112175
Status Public on Mar 12, 2019
Title The long noncoding RNA Lnczc3h7a promotes TRIM25-mediated RIG-I antiviral innate immune response
Organism Mus musculus
Experiment type Other
Summary In this study, we employed a combination of RIP-seq and iCLIP-seq technologies to identify long non-coding RNA associated with cytoplasmic TRIM25 and RIG-I protein in mouse macrophages cells.
Overall design Two UV-RIP experiments were performed using anti-Flag antibody in RAW264.7 cells stably expressing Flag-tagged TRIM25 with or without VSV infection for 8 hr, then co-purified RNAs were analyzed using Illumina Xten. iCLIP experiment was performed using anti-TRIM25 antibody and anti-RIG-I antibody in RAW264.7 cells upon VSV infection for 8 hr and then was analyzed by Illumina HiSeq2500 SE50 sequencing.
Contributor(s) Lin H, Jiang M, Liu L, Yang Z, Ma Z, Liu S, Ma Y, Zhang L, Cao X
Citation(s) 31036902
Submission date Mar 21, 2018
Last update date Jun 12, 2019
Contact name Hongyu Lin
Phone 008613070178987
Organization name Zhejiang University School of Medicine
Street address Zijingang Campus of Zhejiang University, 866 Yuhangtang Road, Xihu District
City hangzhou
ZIP/Postal code 310058
Country China
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM3059339 TRIM25-iCLIP-seq
GSM3059340 RIG-I-iCLIP-seq
GSM3583899 UV-RIP-seq 0h
BioProject PRJNA443151
SRA SRP136203

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112175_0h_8h-isoforms.fpkm_tracking.txt.gz 3.7 Mb (ftp)(http) TXT
GSE112175_RAW.tar 80.0 Kb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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