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| Status |
Public on Mar 12, 2019 |
| Title |
The long noncoding RNA Lnczc3h7a promotes TRIM25-mediated RIG-I antiviral innate immune response |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
In this study, we employed a combination of RIP-seq and iCLIP-seq technologies to identify long non-coding RNA associated with cytoplasmic TRIM25 and RIG-I protein in mouse macrophages cells.
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| Overall design |
Two UV-RIP experiments were performed using anti-Flag antibody in RAW264.7 cells stably expressing Flag-tagged TRIM25 with or without VSV infection for 8 hr, then co-purified RNAs were analyzed using Illumina Xten. iCLIP experiment was performed using anti-TRIM25 antibody and anti-RIG-I antibody in RAW264.7 cells upon VSV infection for 8 hr and then was analyzed by Illumina HiSeq2500 SE50 sequencing.
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| Contributor(s) |
Lin H, Jiang M, Liu L, Yang Z, Ma Z, Liu S, Ma Y, Zhang L, Cao X |
| Citation(s) |
31036902 |
| |
| Submission date |
Mar 21, 2018 |
| Last update date |
Jun 12, 2019 |
| Contact name |
Hongyu Lin |
| E-mail(s) |
helly147@163.com
|
| Phone |
008613070178987
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| Organization name |
Zhejiang University School of Medicine
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| Street address |
Zijingang Campus of Zhejiang University, 866 Yuhangtang Road, Xihu District
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| City |
hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (2) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA443151 |
| SRA |
SRP136203 |
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