|
| Status |
Public on Mar 01, 2019 |
| Title |
Genome-wide assay of BRG1 regulated genes in inflamed colon epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Here we focus on the function of BRG1 in colitis and colitis-associated-cancer, in the aim of finding the direct target genes of BRG1 which may response for the phenotype we observed in BRG1 conditional knock-out mice, ChIP-Seq assay is used. The cells used for ChIP-Seq is colon epithelial cells which isolated from DSS treated mice.
|
| |
|
| Overall design |
To mimic the status of BRG1IEC KO mice, wild-type mice were treated with 2% DSS for 3 days and IECs were isolated by EDTA isolation buffer, and each sample contains pooled IECs from 5 mice. Flash frozen cells were then sent to Active Motif, Inc. for chromatin extraction and followed by immunoprecipitation using anti-BRG1 antibody (Abcam, cat # ab110641).
|
| |
|
| Contributor(s) |
Liu Y, Qin J |
| Citation(s) |
31601814 |
| |
| Submission date |
Mar 21, 2018 |
| Last update date |
Oct 21, 2019 |
| Contact name |
Yongfeng Liu |
| E-mail(s) |
lyf3433@163.com
|
| Organization name |
The Institute of Health Sciences, SIBS, CAS / SJTUSM
|
| Street address |
320 Yueyang Road, Shanghai, 200025 P.R. China
|
| City |
shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE112128 |
Function of BRG1 in colitis and colitis-associated-cancer |
|
| Relations |
| BioProject |
PRJNA439398 |
| SRA |
SRP136165 |
